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Pelczaria aurantia gen. nov., sp. nov., a newly described organge-colored bacterium

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Abstract

An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Grampositive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at room temperature (ca. 22°C); growth was slower and less extensive at 30°C or 37°C. No growth was seen at 4–5°C or at 42°C. It withstands brief exposure to ultraviolet radiation. Its growth was inhibited by low levels (0.1 unit/ml) of penicillin but was unaffected by levels of acetazolamide in excess of 1 mg/ml, indicating that it lacks carbonic anhydrase. Acid was not produced from glucose, maltose, mannose, lactose, or sucrose and only weakly, if at all, from fructose. Its DNA has a G+C mol percent of 59 measured chromatographically and neither the DNA nor rRNA from the organism hybridized with DNA from any organism that seemed related on morphological or other bases. Thin-layer chromatography of chloroform: methanol extracts of the organism show that it contains phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl glycerol. Cell-wall preparations contain glutamic acid, serine, histidine, lysine, and alanine in the ratio of 1:1:1:1:8. Colonies were red-orange in color due, in larger measure, to a carotenoid tentatively identified as rhodopin. The organism was named Pelczaria aurantia.

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References

  1. Anonymous (1953) Difco Manual of dehydrated culture media and reagents for microbiological and clinical laboratory procedures, 9th ed. Difco Laboratories, Detroit, Michigan, p 190

  2. Berger U, Issi R (1971) Resistenz gegen Acetazolamide als taxonomisches Kriterium bei Neisseria. Arch Hyg 154: 540–544

  3. Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using pre-column derivatization. J Chromatogr 336: 93–104

  4. Buck JD (1982) Non staining (KOH) method for determination of Gram reactions of Marine bacteria. Appl Environ Microbiol 44: 992–993

  5. Collins CH, Lyne PM (1970) Microbiological methods, 3rd edn. Butterworths-University Park Press, Baltimore, MD. pp. 147, 168

  6. Conn HJ, Bartholomew JW, Jennison MW (1957a) Staining methods. In: Pelczar MJ (ed) Manual of microbiological methods, McGraw-Hill Book Company, New York, pp. 10–36

  7. Conn HJ, Jennison MW, Weeks OB (1957b) Routine-tests for the identification of bacteria. In: Pelczar MJ (ed) Manual of microbiological methods, McGraw-Hill Book Company, Inc., New York, pp 140–168

  8. D'Amato RR, Eriquez LA, Tomfohrde KM, Singerman E (1978) Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles. J Clin Microbiol 7: 77–81

  9. Hoke C, Vedros NA (1982) Taxonomy of the Neisseria: fatty acid analysis, aminopeptidase activity and pigment extraction. Int J Syst Bacteriol 32: 51–56

  10. Johnson JL (1985) DNA reassociation and RNA hybridisation of bacterial nucleic acids. Methods Microbiol 18: 33–74

  11. Komagata K, Suzuki K-I (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 191–207

  12. Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118

  13. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167

  14. Nelis HJ, DeLeenheer AP (1989) Profiling and quantitation of bacterial carotenoids by liquid chromatography and photodiode array detection. Appl Environ Microbiol 55: 3065–3071

  15. Work E (1971) Cell walls. Methods Microbiol 5A: 361–418

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This paper is submitted in honor of Miss Dorothy Schoknecht of Kalispell, Montana, teacher prima inter pares

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Poston, J.M. Pelczaria aurantia gen. nov., sp. nov., a newly described organge-colored bacterium. Arch. Microbiol. 160, 114–120 (1993). https://doi.org/10.1007/BF00288712

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Keywords

  • Fructose
  • Carotenoid
  • Lactose
  • Glutamic Acid
  • Maltose