The interspersion period of repetitive and unique sequences was analyzed by two different methods, electron microscopy and agarose gel electrophoresis, for four Amphibian species with different nuclear DNA content, namely the Anura Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) and the Urodela Triturus cristatus (23 pg) and Necturus maculosus (52 pg). Within each of the two subclasses it has been found that interspecific differences, in DNA content, due to variations in the amount of repetitive sequences, do not involve variations in length of the interspersed repetitive sequences. They remain about 380 base pairs. Furthermore, the unique sequences length has been found to be shorter in Bufo (760 base pairs) than in Xenopus (1600) and in Necturus (880) than in Triturus (1340). A study of the interspersion period has shown that the great difference in DNA content between Anura and Urodela, which had been previously shown not to have involved changes in the relative amounts of the various sequence classes, does not involve changes in the interspersion period.
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Baldari, C.T., Amaldi, F.: DNA reassociation kinetics in relation to genome size in four Amphibian species. Chromosoma (Berl.) 59, 13–22 (1976)
Britten, R.J., Davidson, E.H.: Gene regulation for higher cells: a theory. Science 165, 349–357 (1969)
Buongiorno-Nardelli, M., Amaldi, F., Beccari, E., Junakovic, N.: Size of ribosomal DNA repeating units in Xenopus laevis: limited individual heterogeneity and extensive population polymorphism. J. molec. Biol. 109, 105–117 (1977)
Chamberlin, M.E., Britten, R.J., Davidson, E.H.: Sequence organization in Xenopus DNA studied by the electron microscope. J. molec. Biol. 96, 317–333 (1975)
Davidson, E.H., Galau, G.A., Angerer, R.C., Britten, R.J.: Comparative aspects of DNA organization in Metazoa. Chromosoma (Berl.) 51, 253–259 (1975)
Davidson, E.H., Graham, D.E., Neufeld, B.R., Chamberlin, M.E., Amenson, C.S., Hough, B.R., Britten, R.J.: Arrangement and characterization of repetitive sequence elements in animal DNAs. Cold Spr. Harb. Symp. quant. Biol. 38, 295–301 (1974)
Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with non repetitive sequence elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973)
Davis, R., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. 21, pp. 413–428. New York: Academic Press 1971
Flavell, R.B., Bennet, M.D., Smith, J.B., Smith, D.B.: Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem. Genet. 12, 257–269 (1974)
Manning, J.E., Schmid, C.W., Davidson, N.: Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell 4, 141–155 (1975)
Mizuno, S., MacGregor, H.C.: Chromosomes, DNA sequences and evolution in Salamanders of the genus Plethodon. Chromosoma (Berl.) 48, 239–296 (1974)
Morescalchi, A., Serra, V.: DNA renaturation kinetics in some paedogenetic Urodeles. Experientia (Basel) 30, 487–489 (1974)
Narayan, R.K.J., Rees, H.: Nuclear DNA variation in Lathyrus. Chromosoma (Berl.) 54, 141–154 (1976)
Straus, N.A.: Comparative DNA renaturation kinetics in Amphibians. Proc. nat. Acad. Sci. (Wash.) 68, 799–802 (1971)
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Baldari, C.T., Amaldi, F. Length and interspersion of repetitive and non repetitive DNA sequences in four Amphibian species with different genome sizes. Chromosoma 61, 359–368 (1977). https://doi.org/10.1007/BF00288619
- Base Pair
- Developmental Biology
- Genome Size
- Sequence Length
- Repetitive Sequence