Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

A simple procedure for obtaining autoradiographs of G-banded chromosomes


Chromosomal preparations from cells flash-labeled with 3H-dT were Giemsa-banded following trypsin digestion, allowed to air-dry, and then were coated with a layer of 1% Formvar. The slides were subsequently coated with radiographic emulsion (NTB2) and processed for autoradiography. The resulting chromosomes had distinct G-banding and radiographic labeling patterns. Chemographic grain formation in the emulsion, normally caused by Giemsa stain, was prevented by the film of Formvar, allowing a very low background grain level.

This is a preview of subscription content, log in to check access.


  1. Deaven, L.L., Petersen, D.F.: The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analysis. Chromosoma (Berl.) 41, 129–144 (1973)

  2. Schneider, L.K., Pierce, S.L.: An assessment of routine chromosomal staining procedures in radioautography. Stain Technol. 48, 69–76 (1973)

  3. Seabright, M.: A rapid banding technique for human chromosomes. Lancet 1971 II, 971–972

  4. Stubblefield, E.: The kinetics of DNA replication in chromosomes. In: The cell nucleus, (H. Busch, ed.), Vol. 2, pp. 149–162. New York: Academic Press 1974

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rutledge, M.H. A simple procedure for obtaining autoradiographs of G-banded chromosomes. Chromosoma 70, 259–262 (1979).

Download citation


  • Trypsin
  • Developmental Biology
  • Simple Procedure
  • Trypsin Digestion
  • Chromosomal Preparation