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A simple procedure for obtaining autoradiographs of G-banded chromosomes

Abstract

Chromosomal preparations from cells flash-labeled with 3H-dT were Giemsa-banded following trypsin digestion, allowed to air-dry, and then were coated with a layer of 1% Formvar. The slides were subsequently coated with radiographic emulsion (NTB2) and processed for autoradiography. The resulting chromosomes had distinct G-banding and radiographic labeling patterns. Chemographic grain formation in the emulsion, normally caused by Giemsa stain, was prevented by the film of Formvar, allowing a very low background grain level.

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References

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Rutledge, M.H. A simple procedure for obtaining autoradiographs of G-banded chromosomes. Chromosoma 70, 259–262 (1979). https://doi.org/10.1007/BF00288410

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Keywords

  • Trypsin
  • Developmental Biology
  • Simple Procedure
  • Trypsin Digestion
  • Chromosomal Preparation