Divergent pattern in pyruvate efflux from livers perfused with As2O3 and livers of animals previously repeatedly treated with the toxicant was observed in earlier experiments (Reichl et al. 1987, 1988). Further studies of the effect of As2O3 on carbohydrate metabolism were therefore performed. Male guinea pigs received either a single dose of As2O3 10 mg· kg−1 s.c. or repeated doses of 2.5 mg·kg−1 bis in die (b.i.d.) on 5 consecutive days. One hour after the single dose or 1 h and 16 h after the last injection in the repeated treatment group the animals were sacrificed under anaesthesia. The livers were removed by a freeze-stop technique and the contents of glycogen and glycolysis intermediates were measured. In the single dose group a decrease in fructose-1,6-diphosphate and glycerolaldehyde-3-phosphate and an increase in phosphoenolpyruvate and pyruvate was observed. In the repeat dose, 1-h group a significant decrease in glycogen, glucose-6-phosphate, fructose-6-phosphate, glycerolaldehyde-3-phosphate, dihydroxyacetonephosphate, 2-phosphoglycerate and pyruvate was found. In the repeat dose, 16-h group the contents of glycogen, glucose-6-phosphate, pyruvate and lactate were diminished. The most prominent finding after repeated As2O3 administration was a marked depletion in total carbohydrate content. This was due mainly to depletion of glycogen.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bergmeyer HU (1984) Methods of enzymatic analysis; metabolites 1: carbohydrate, 6. Verlag Chemie, Weinheim
Berry LJ, Smythe DS (1959) Carbohydrate metabolism in normal and altitude-exposed mice following arsenite poisoning. Am J Physiol 197: 37–40
Brown MM, Rhyne BC, Goyer RA, Fowler BA (1976) Intracellular effects of chronic arsenic administration on renal proximal tubule cells. J Toxicol Environ Health 1: 505–514
Crema A (1955) Distribution et élimination de l'arsenic 76 chez la souris normale et cancéreuse. Arch Int Pharmacodyn 103: 57–70
Fluharty AL, Sanadi DR (1960) Evidence for a vicinal dithiol in oxidative phosphorylation. Proc Natl Acad Sci 46: 608–616
Fluharty AL, Sanadi DR (1961) On the mechanism of oxidative phosphorylation. II. Effects of arsenite alone and in combination with 2,3 dimercaptopropanol. J Biol Chem 236: 2772–2778
Mealey J, Brownell GL, Sweet WH (1959) Radioarsenic in plasma, urine normal tissues and intracranial neoplasms. Arch Neurol Psychiatr 81: 310–320
Mohelska H, Bencko V, Smetana K, Hyncica V (1980) Ultrastructrual changes in hepatocytes of mice exposed to arsenic drinking water. Exp Pathol (Jena) 18(5): 275–281
Peters RA (1955) Biochemistry of some toxic agents. 1. Present state of knowledge of biochemical lesions induced by trivalent arsenical poisoning. Bull John Hopkins Hosp 97: 1–20
Reichl FX, Szinicz L, Kreppel H, Forth W (1987) Glucose metabolism in perfused livers of guinea pigs repeatedly treated with As2O3. Naunyn Schmiedeberg's Arch Pharm 335 (suppl): R17
Reichl FX, Szinicz L, Kreppel H, Fichtl B, Forth W (1988) Effect of As2O3 on carbohydrate metabolism after single or repeated injection in guinea pigs. Toxicologist 8(1): 20
Sachs L (1978) Angewandte Statistik; statistische Methoden und ihre Anwendung. Springer-Verlang, Berlin Heidelberg New York
Szinicz L, Forth W (1988) Effect of As2O3 on gluconeogenesis. Arch Toxicol 61: 444–449
Vahter M, Norin H (1980) Metabolism of 74As-labelled trivalent and pentavalent inorganic arsenic. Environ Res 21: 446–457
Wollenberger A, Ristau O, Schoffa G (1960) Eine einfache Technik der extrem schnellen Abkühlung größerer Gewebestücke. Pflügers Arch 270: 339–412
About this article
Cite this article
Reichl, F., Szinicz, L., Kreppel, H. et al. Effect of arsenic on carbohydrate metabolism after single or repeated injection in guinea pigs. Arch Toxicol 62, 473–475 (1988). https://doi.org/10.1007/BF00288353
- Guinea pigs