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The grasshopper X chromosome

I. States of condensation and the nuclear envelope at G1, S and G2 of premeiotic interphase and at early meiotic prophase

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Abstract

The sub-stages of spermatocyte interphase (G1, S and G2) have been identified in the grasshopper Brachystola magna using E.M. autoradiography and serial thin sectioning techniques. The X chromosome occupies a nuclear envelope bound compartment separate from an autosome compartment during G1 and S. At G2 the X compartment is resolved by coalescence of the membranes enveloping the X chromosome and autosome compartments. — At G1 and S, the compartmentalized X chromatin is laced with nuclear membrane material. This X chromatin associated membrane decreases in amount as the cell passes through G2 and enters early meiotic prophase. There are at least 2 and possibly 3 states of condensation of the heterochromatic X during premeiotic interphase and early meiotic prophase correlated with the presence or absence of membrane material associated with the chromatin. — The X chromatin replicates asynchronously with autosomal euchromatin and synchronously with autosomal heterochromatin associated with nucleoli. The X chromatin replication appears to be associated with the nuclear membrane. — The observations indicate that the nuclear membrane is involved with X chromosome condensation and may be implicated in asynchronous X chromosome replication as well.

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References

  1. Church, K.: Arrangement of chromosome ends and axial core formation during early meiotic prophase in the male grasshopper Brachystola magna by 3D, E.M. reconstruction. Chromosoma (Berl.) 58, 365–376 (1976)

  2. Church, K.: Chromosome ends and the nuclear envelope at premeiotic interphase in the male grasshoper Brachystola magna by 3D, E.M. reconstruction. Chromosoma (Berl.) 64, 143–154 (1977)

  3. Comings, D.E.: The role of heterochromatin. In: Birth defects (A.G. Motulsky and W. Lentz, eds.), pp. 44–52. Amsterdam: Excerpta Med. (1974)

  4. Cook, P.R.: On the inheritance of differentiated traits. Biol. Rev. 49, 51–84 (1974)

  5. Edenberg, H.J., Huberman, J.A.: Eukaryotic chromosome replication, Ann. Rev. Genet. 9, 245–284 (1975)

  6. Fox, D.P., Hewitt, G.M., Hall, D.J.: DNA Replication and RNA transcription of euchromatic and heterochromatic chromosome regions during grasshopper meiosis. Chromosoma (Berl.) 45, 42–62 (1974)

  7. Henderson, S.A.: RNA synthesis during male meiosis and spermeogenesis. Chromosoma (Berl.) 15, 345–366 (1964)

  8. Lifschytz, E., Lindsley, D.L.: The Role of X chromosome inactivation during spermatogenesis. Proc. nat. Acad. Sci. (Wash.) 69, 182–186 (1972)

  9. Lima-de-Faria, A.: Differential uptake of tritiated thymidine into hetero- and euchromatin in Melanoplus and Secale. J. biophys. biochem. Cytol. 6, 457–466 (1959)

  10. Lyon, M.F.: X-chromosome inactivation and developmental patterns in mammals. Biol. Rev. 47, 1–35 (1972)

  11. Moens, P.B.: Serial Sectioning in electron microscopy. Proc. Canad. Fed. Biol. Soc. 13, 160 (1970)

  12. Monesi, V.: Differential Rate of ribonucleic acid synthesis in the autosomes and sex chromosomes during male meiosis in the mouse. Chromosoma (Berl.) 17, 11–21 (1965)

  13. Morishama, A., Grumbach, M.M., Taylor, J.H.: Asynchronous duplication of human chromosomes and the origin of sex chromatin. Proc. nat. Acad. Sci. (Wash.) 48, 756–763 (1962)

  14. Nicklas, R.B., Jaqua, R.A.: X-chromosome DNA replication developmental shift from synchrony to asynchrony. Science 147, 1041–1043 (1965)

  15. Sutton, W.S.: The spermatogonial divisions in Brachystola magna. Kansas Univ. Quart. 9, 135–159 (1900)

  16. White, M.J.D.: The heteropycnosis of sex chromosomes and its interpretation in terms of spiral structure. J. Genet. 40, 67–81 (1940)

  17. White, M.J.D.: Asymmetry of heteropycnosis in tetraploid cells of a grasshopper. Chromosoma (Berl.) 30, 51–61 (1970)

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Church, K. The grasshopper X chromosome. Chromosoma 71, 347–358 (1979). https://doi.org/10.1007/BF00287140

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Keywords

  • Developmental Biology
  • Thin Section
  • Nuclear Envelope
  • Nuclear Membrane
  • Membrane Material