We have developed a new enzyme-linked immunosorbent assay for determination of H-Y phenotype in the human. This assay, which measures the inhibition of the reaction of a monoclonal anti-H-Y antibody and a mouse testis extract as a source of H-Y antigen, was applied to the supernatant of lymphocytes from ten normal male and ten normal female subjects. Introduction of supernatant from male cells gave reading of 69%–78% of those obtained with testis supernatant alone; female-cell supernatant did not inhibit the reaction (89%–102%).
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Farber, C., Liebenthal, D., Wachtel, S.S. et al. Detection of H-Y in the enzyme-linked immunosorbent assay. Hum Genet 65, 278–279 (1984). https://doi.org/10.1007/BF00286517
- Internal Medicine
- Metabolic Disease
- Immunosorbent Assay
- Female Subject
- Male Cell