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Detection of H-Y in the enzyme-linked immunosorbent assay


We have developed a new enzyme-linked immunosorbent assay for determination of H-Y phenotype in the human. This assay, which measures the inhibition of the reaction of a monoclonal anti-H-Y antibody and a mouse testis extract as a source of H-Y antigen, was applied to the supernatant of lymphocytes from ten normal male and ten normal female subjects. Introduction of supernatant from male cells gave reading of 69%–78% of those obtained with testis supernatant alone; female-cell supernatant did not inhibit the reaction (89%–102%).

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Correspondence to Claire-Michele Farber.

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Farber, C., Liebenthal, D., Wachtel, S.S. et al. Detection of H-Y in the enzyme-linked immunosorbent assay. Hum Genet 65, 278–279 (1984).

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  • Internal Medicine
  • Metabolic Disease
  • Immunosorbent Assay
  • Female Subject
  • Male Cell