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Continuous culture of Candida utilis: influence of medium nitrogen concentration

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When Candida utilis was grown in continuous culture, decreasing the concentration of N in the medium affected cell composition, biomass yield, biomass productivity, maximal growth rate and cell morphology. When the dilution rate was low (0.1 h-1), reducing N from 1100 to 100 mg/l led to a 40% decrease in RNA content of the cells. Nitrogen-limited growth, which occurred when N<420 mg/l, was associated with significant changes in cell-wall carbohydrates and a significant reduction in the glycogen content of the cells. A set of culture conditions was established which permitted maximal consumption of the main nutrients in the medium and the production of yeast biomass suitable as a source of single-cell protein.

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  1. Alroy, Y. & Tannenbaum, S.R. 1973 The influence of environmental conditions on the macromolecular composition of Candida utilis. Biotechnology and Bioengineering, 15, 239–256.

  2. Callieri, D.A.S., Nuñez, C.G., Díaz Ricci, J.C. & Scidá, L.A. 1984 Batch culture of Candida utilis in a medium deprived of phosphorous source. Applied Microbiology and Biotechnology 19, 267–271.

  3. Dawson, P.S.S. & Steinhauer, L.P. 1980 Dynamic aspects of radiorespirometry during cell cycle of Candida utilis: some observations under carbon, nitrogen and phosphorus-limited growth. Biotechnology and Bioengineering 22, 137–156.

  4. Díaz Ricci, J.C., Callieri, D.A., Núñez, C.G. & Scidá, L.A. 1983 Desarrollo de Candida utilis en un medio simple con distintos niveles limitantes de glucosa. Acta Científica Venezolana 34, 341–344.

  5. Dubowsky, K.M. 1962 An o-toluidine method for body fluid glucose determination. Clinical Chemistry 8, 215.

  6. Herbert, D. 1958 Continuous culture of microorganisms: some theoretical aspects. In Continuous Cultivation of Microorganisms. A Symposium, pp. 45–52. Prague: Czechoslovak Academy of Sciences.

  7. Lindberg, O. & Ernster, L. 1955 Determination of inorganic phosphorus compounds by phosphate analysis. Methods in Biochemical Analysis 3, 1–22.

  8. Lucca, M.E., Romero, M.E., Díaz Ricci, J.C., Garro, O.A. & Callieri, D.A.S. 1991 Composition and morphology of Candida utilis grown in continuous culture with decreasing concentrations of phosphate. World Journal of Microbiology and Biotechnology 7, 359–364.

  9. Málek, I. 1958 The physiological state of microorganisms during continuous culture. In Continuous Cultivation of Microorganisms. A Symposium, pp. 11–28. Prague: Czechoslovak Academy of Sciences.

  10. Pirt, S.J. 1975 Energy and carbon source requirements. In Principles of Microbe and Cell Cultivation, pp. 63–80. London: Blackwell Scientific.

  11. Sacktor, B. & Hurlbut, E.C. 1966 Regulation of metabolism in working muscle in vivo. II. Concentrations of adenine nucleotides, arginine, phosphate, and inorganic phosphate in insect flight muscle during flight. Journal of Biological Chemistry 241, 632–638.

  12. Soumalainen, H. & Oura, E. 1975 Yeast nutrition and solute uptake. In Physiology and Biochemistry of Yeasts, Vol. 2, 2nd edn, eds Rose, A.H. & Harrison, J.S. London: Academic Press.

  13. Stewart, P.R. 1975 Analytical methods for yeasts. Methods in Cell Biology 12, 112–114.

  14. Trevelyan, W.E. & Harrison, J.S. 1952 Studies on yeast metabolism. 1. Fractionation and microdetermination of cell carbohydrates. Biochemical Journal 50, 298–303.

  15. Trevelyan, W.E. & Harrison, J.S. 1956 Yeast carbohydrates fractions separation from nucleic acid analysis and behaviour during anaerobic fermentation. Biochemical Journal 63, 23–33.

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Correspondence to M. E. Lucca.

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Lucca, M.E., Romero, M.E. & Callieri, D.A.S. Continuous culture of Candida utilis: influence of medium nitrogen concentration. World J Microbiol Biotechnol 11, 515–518 (1995). https://doi.org/10.1007/BF00286365

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Key words

  • Candida utilis
  • chemostat
  • nitrogen metabolism
  • phosphate
  • single-cell protein