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Continuous culture of Candida utilis: influence of medium nitrogen concentration

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Abstract

When Candida utilis was grown in continuous culture, decreasing the concentration of N in the medium affected cell composition, biomass yield, biomass productivity, maximal growth rate and cell morphology. When the dilution rate was low (0.1 h-1), reducing N from 1100 to 100 mg/l led to a 40% decrease in RNA content of the cells. Nitrogen-limited growth, which occurred when N<420 mg/l, was associated with significant changes in cell-wall carbohydrates and a significant reduction in the glycogen content of the cells. A set of culture conditions was established which permitted maximal consumption of the main nutrients in the medium and the production of yeast biomass suitable as a source of single-cell protein.

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Correspondence to M. E. Lucca.

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Lucca, M.E., Romero, M.E. & Callieri, D.A.S. Continuous culture of Candida utilis: influence of medium nitrogen concentration. World J Microbiol Biotechnol 11, 515–518 (1995). https://doi.org/10.1007/BF00286365

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Key words

  • Candida utilis
  • chemostat
  • nitrogen metabolism
  • phosphate
  • single-cell protein