Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The formation of polytene chromosomes during macronuclear development of the hypotrichous ciliate Stylonychia mytilus

  • 53 Accesses

  • 31 Citations

Abstract

The formation of polytene chromosomes during macronuclear development of the ciliate Stylonychia mytilus was examined in spread electron microscopical preparations. The chromatin organization of early macronuclear anlagen closely resembles the organization of micronuclear chromatin. In the course of polytenization 300 Å chromatin fibers become organized in loop-like structures laterally attached to a thinner axial fiber. It is suggested that this reorganization of chromatin during polytenization is a necessary event for the subsequent chromatin elimination.

This is a preview of subscription content, log in to check access.

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

References

  1. Ammermann, D.: Morphology and development of the macronuclei of the ciliates Stylonychia mytilus and Euplotes aediculatus. Chromosoma (Berl.) 33, 209–237 (1971)

  2. Ammermann, D., Steinbrück, G., Berger, L. v., Hennig, W.: The development of the macronucleus in the ciliated protozoan Stylonychia mytilus. Chromosoma (Berl.) 45, 401–429 (1974)

  3. Elsevier, S.M., Lipps, H.J., Steinbrück, G.: Histone genes in macronuclear DNA of the ciliate Stylonychia mytilus. Chromosoma (Berl.) 69, 291–306 (1978)

  4. Lawn, R.M., Herrick, G., Heumann, J., Prescott, D.M.: Structural organization of the gene-sized pieces of DNA in the ciliate Oxytricha. Cold Spr. Harb. Symp. quant. Biol. 42, 483–492 (1978)

  5. Lipps, H.J., Steinbrück, G.: Free genes for rRNAs in the macronuclear genome of the ciliate Stylonychia mytilus. Chromosoma (Berl.) 69, 21–26 (1978)

  6. Lipps, H.J., Nock, A., Riewe, M., Steinbrück, G.: Chromatin structure of the macronucleus of Stylonychia mytilus. Nucleic Acids Res. 5, 4699–4709 (1978)

  7. Meyer, G.F., Lipps, H.J.: Chromatin elimination in the hypotrichons ciliate Stylonychia mytilus. Chromosoma (Berl.) 77, 285–297 (1980)

  8. Meyer, G.F., Renz, M.: Native and reconstituted chromosome fiber fragments. Chromosoma (Berl.) 75, 177–184 (1979)

  9. Prescott, D.M., Bostock, C.J., Murti, K.G., Lauth, H.R., Gamov, E.: DNA of ciliated protozoa. I. Electron microscopic and sedimentation analysis of macronuclear and micronuclear DNA of Stylonychia mytilus. Chromosoma (Berl.) 34, 355–366 (1971)

  10. Prescott, D.M., Murti, K.G., Bostock, C.J.: Genetic apparatus of Stylonychia sp. Nature (Lond.) 242, 597–600 (1973)

Download references

Author information

Additional information

Dedicated to Professor W. Beermann on the occasion of his 60th birthday

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Meyer, G.F., Lipps, H.J. The formation of polytene chromosomes during macronuclear development of the hypotrichous ciliate Stylonychia mytilus . Chromosoma 82, 309–314 (1981). https://doi.org/10.1007/BF00286113

Download citation

Keywords

  • Developmental Biology
  • Polytene Chromosome
  • Axial Fiber
  • Chromatin Organization
  • Chromatin Fiber