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Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster

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Primary cell cultures derived from mutants in seventeen different genes were analyzed for their ability to excise pyrimidine dimers from DNA. Five of these mutagen-sensitive mutants [mus(2)205A1, mus(3)302D1, mus(3) 304D3, mus(3)306D1, mus(3)308D2] display a significantly reduced excision capacity relative to control cultures. In addition, two of the five [mus(3)306D1, mus(3)308D2] are defective in the accumulation of single-strand breaks normally seen after ultraviolet irradiation. This study, therefore, brings the total number of Drosophila mutants known to be defective in excision repair to seven. The results are discussed relative to other genetic and biochemical properties of these mutants.

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ultraviolet irradiation


methyl methanesulfonate


nitrogen mustard (mechlorethamine)


  1. Baker, B.S., Boyd, J.B., Carpenter, A.T.C., Green, M.M., Nguyen, T.D., Ripoll, P., Smith, P.D.: Genetic controls of meiotic recombination and somatic DNA metabolism in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 73, 4140–4144 (1976)

  2. Beukers, R., Berends, W.: Isolation and identification of the irradiation product of thymine. Biochim. Biophys. Acta (Amst.) 41, 550–551 (1960)

  3. Boyce, R.P., Howard-Flanders, P.: Release of ultraviolet light-induced thymine dimers from DNA in E. coli K-12. Proc. nat. Acad. Sci. (Wash.) 51, 293–300 (1964)

  4. Boyd, J.B., Golino, M.D., Setlow, R.B.: The mei-9a mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. Genetics 84, 527–544 (1976)

  5. Boyd, J.B., Harris, P.V., Osgood, C.J., Smith, K.E.: Biochemical characterization of repair-deficient mutants of Drosophila. In: DNA repair and mutagenesis in eukaryotes (W.M. Generoso, M.D. Shelby and F.J. de Serres, eds.), pp. 209–221. New York: Plenum Press 1980

  6. Boyd, J.B., Setlow, R.B.: Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster. Genetics 84, 507–526 (1976)

  7. Buhl, S.N., Setlow, R.B., Regan, J.D.: DNA repair in Potorous tridactylus. Biophys. J. 14, 791–803 (1974)

  8. Cleaver, J.E.: Defective repair replication of DNA in xeroderma pigmentosum. Nature (Lond.) 218, 652–656 (1968)

  9. Dunn, W.C., Regan, J.D.: Inhibition of DNA excision repair in human cells by arabinofuranosyl cytosine: Effect on normal and xeroderma pigmentosum cells. Molec. Pharmacol. 15, 367–374 (1979)

  10. Hanawalt, P.C., Cooper, P.K., Ganesan, A.K., Smith, C.A.: DNA repair in bacteria and mammalian cells. Ann. Rev. Biochem. 48, 783–836 (1979)

  11. Harris, P.V., Boyd, J.B.: Excision repair in Drosophila. Analysis of strand breaks appearing in DNA of mei-9 mutants following mutagen treatment. Biochim. biophys. Acta (Amst.) 610, 116–129 1981)

  12. Keijzer, W., Jaspers, N.G.J., Abrahams, P.J., Taylor, A.M.R., Arlett, C.F., Zelle, B., Takebe, H., Kinmont, P.D.S., Bootsma, D.: A seventh complementation group in excision-deficient xeroderma pigmentosum. Mutation Res. 62, 183–190 (1979)

  13. Kohn, K.W., Erickson, L.C., Ewig, R.A.G., Friedman, C.A.: Fractionation of DNA from mammalian cells by alkaline elution. Biochemistry 15, 4629–4637 (1976)

  14. Lehmann, A.R., Kirk-Bell, S., Arlett, C.F., Harcourt, S.A., de Weerd-Kastelein, E.A., Keijzer, W., Hall-Smith, P.: Repair of ultraviolet light damage in a variety of human fibroblast cell strains. Cancer Res. 37, 904–910 (1977)

  15. Lindsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Washington Publ. 647, 1980

  16. Pettijohn, D., Hanawalt, P.: Evidence for repair replication of ultraviolet damaged DNA in bacteria. J. molec. Biol. 9, 395–410 (1964)

  17. Reynolds, R.J.: Removal of pyrimidine dimers from Saccharomyces cerevisiae nuclear DNA under nongrowth conditions as detected by a sensitive enzymatic assay. Mutation Res. 50, 43–56 (1978)

  18. Setlow, R.B., Carrier, W.L.: The disappearance of thymine dimers from DNA: An error-correcting mechanism. Proc. nat. Acad. Sci. (Wash.) 51, 226–231 (1964)

  19. Smith, P.D., Snyder, R.D., Dusenberg, R.L.: Isolation and characterization of repair-deficient mutants of Drosophila melanogaster. In: DNA repair and mutagenesis in eukaryotes (W.M. Generoso, M.D. Shelby, F.J. de Serres, eds.), pp. 175–188. New York: Plenum Press 1980

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This work is dedicated to Professor W. Beermann whose own contributions were instrumental in focusing a modern analysis of the eukaryotic genome on the diptera. Those of us who benefitted so much from his personal guidance recognize that we did so as a result of some sacrifice on his part. One of Boyd's contemporaries in Tübingen once remarked: “It's terrifying to think what Professor Beermann could do if he were in the lab full time.”

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Boyd, J.B., Harris, P.V. Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster . Chromosoma 82, 249–257 (1981). https://doi.org/10.1007/BF00286109

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  • Cell Culture
  • Developmental Biology
  • Pyrimidine
  • Primary Cell
  • Ultraviolet Irradiation