Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Regeneration and rapid multiplication of Bowiea volubilis Harv. in tissue culture


Callus cultures were raised from segments of inflorescence axis of Bowies volubilis Harv. (Liliaceae) on a modified basal medium of Murashige and Skoog (1962) (MS) supplemented with 1 mg 1−1 2,4-dichlorophenoxy acetic acid (2,4-D) + 15% v/v coconut milk. Shoot primordia developed after 2–3 subcultures when auxin concentration was lowered. Rooted bulbous plants were obtained in MS medium without any hormone.

Shoots were induced directly on scales of regenerated bulbs used as secondary explants on modified MS medium supplemented with 2 mg 1−1 6-benzylaminopurine (BAP) + 0.05 mg 1−1 2,4-D. These shoots grew and multiplied rapidly in shake culture using liquid MS medium. From each scale, 400–600 bulblets could be produced in 16–20 weeks period. Eighty percent of plants have survived on transferring to potted soil.

This is a preview of subscription content, log in to check access.


  1. Hussey G (1980) In: Cutler DF & Gregory M (ed) Petaloid Monocotyledons, Academic Press pp 33–42

  2. Hussey G (1982a) In: Fujiwara A (ed) Plant Tissue Culture 1982 Procd 5th Int Cong Plant Tissue & Cell Culture Tokyo pp 677–680

  3. Hussey G (1982b) Ann Bot 49: 407–409

  4. Hussey G, Falavigna A (1980) J exp Bot 31: 1675–1686

  5. Levy LW (1981) Environ Expt Bot 21: 389–395

  6. Jha S, Sen S (1984) Plant Cell Tissue & Organ Culture 3: 91–100

  7. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497

  8. Seabrook JEA, Cumming BG (1977) In vitro 13: 831–836

  9. Simmonds JA, Cumming BG (1976) Scientia Hort 5: 161–170

  10. Takayama S, Misawa M (1983) Can J Bot 61: 224–228

  11. Watt JM, Breyer-Brandwizk MG (1962) Medicinal and Poisonous plants of Southern and Eastern Africa. E & S Livingstone Ltd, Edinburgh & London pp 691–695

Download references

Author information

Correspondence to Sumitra Sen.

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Jha, S., Sen, S. Regeneration and rapid multiplication of Bowiea volubilis Harv. in tissue culture. Plant Cell Reports 4, 12–14 (1985).

Download citation


  • Acetic
  • Acetic Acid
  • Tissue Culture
  • Basal Medium
  • Callus Culture