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Serial sectioning study of some meiotic stages in Scaptericus borrelli (Grylloidea)

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Abstract

An electron microscope study of the spermatocytes I of Scaptericus borrelli was performed using serial sections and tridimensional reconstruction. The stages examined were: mid-pachytene, early diplotene and the so-called “diffuse stage”. At mid-pachytene the nucleolus (associated to an autosome) shows a glomerular array formed of supernumerary synaptonemal complexes (extra-complexes, ECs). At early diplotene the autosomes still show remnants of synaptonemal complexes (SC) and the sex chromosome is formed by two substances: one is homogeneous (chromatin-like) the other is granular and dense. A short number of ECs occur in the latter. — At the “diffuse stage” (“lobulated” in this paper) the SCs remnants and the ECs have disappeared and the granular material is assembled in a nuclear lobule. This lobule was found associated with one centriole. — A tridimensional model of a diplotene nucleus and models of diplotene bivalents are shown. — The capacity to form repetitive components of the synaptonemal complex type by spermatocytes of the super-Family Grylloidea is discussed.

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References

  1. Baker, T. G., Franchi, L. L.: The fine structure of oogonia and oocytes in human ovaries. J. Cell Sci. 2, 213–224 (1967).

  2. Barry, E. G.: The diffuse diplotene stage of meiotic prophase in Neurospora. Chromosoma (Berl.) 26, 119–129 (1969).

  3. Camenzind, R., Nicklas, R. B.: The non-random chromosome segregation in spermatocytes of Gryllotalpa haxadactyla. A micromanipulation analysis. Chromosoma (Berl.) 24, 324–335 (1968).

  4. Coleman, J. R., Moses, M. J.: DNA and the fine structure of synaptic chromosomes in the domestic rooster Gallus domesticus. J. Cell Biol. 23, 63–78 (1964).

  5. Comings, D. E., Okada, T. A.: Whole-mount electron microscopy of meiotic chromosomes and the S.Cs. Chromosoma (Berl.) 30, 269–286 (1970).

  6. Cousin, G.: Biométrie et définition de morphologie quantitative des spèces et de leurs hybrids. Bull. Soc. Zool. France 81, 247–262 (1956).

  7. De Robertis, E. D. P., Nowinski, W. W., Saez, F. A.: Cell biology, 5th edit. Philadelphia, Pa.: W. B. Saunders Co. 1970.

  8. Dingle, A. D., Fulton, Ch.: Development of the flagellar apparatus of Naegleria. J. Cell Biol. 31, 43–54 (1966).

  9. Esponda, P., Stockert, J. C.: Localization of RNA in synaptinemal complex. J. Ultrastruct. Res. 35, 411–417 (1971).

  10. Esponda, P., Stockert, J. C.: Evolution of the synaptinemal complex in Helix aspersa spermatocytes. Chromosoma (Berl.) 36, 150–157 (1972).

  11. Galien, L.: Problèmes et concepts de l'embryologie expérimentale, 2ème. édit. Paris: Librairie Gallimard 1958.

  12. Gall, J. C., Macgregor, H. C., Kidston, M. E.: Gene amplification in the oocytes of dytiscid water beetles. Chromosoma (Berl.) 26, 169–187 (1969).

  13. Gassner, G.: Synaptinemal complexes: recent findings. J. Cell Biol. 35, 166A-167A (1967).

  14. Gassner, G.: Synaptinemal complexes in the achiasmatic spermatogenesis of Bolbe nigra Giglio-Tos (Mantoidea). Chromosoma (Berl.) 26, 22–34 (1969).

  15. Guénin, H. A.: Observations sur la structure submicroscopique du complexe axial dans les chromosomes méiotiques chez Gryllus campestris L. et Gryllus bimaculatus de Geer (Orthopt. Gryll.). J. Microscopie 4, 749–758 (1965).

  16. Jaworska, H., Lima-de-Faría, A.: Multiple synaptinemal complexes at the region of gene amplification in Acheta. Chromosoma (Berl.) 28, 309–327 (1969).

  17. Karlsson, U.: Three dimensional studies of neurons in the lateral geniculate nucleus of the rat. I. Organelle organization in the perikaryon and its proximal branches. J. Ultrastruct. Res. 16, 429–481 (1966a).

  18. Karlsson, U.: Three dimensional studies of neurons in the lateral geniculate nucleus of the rat. II. Environment of perikarya and proximal parts of their branches. J. Ultrastruct. Res. 16, 482–504 (1966b).

  19. Karlsson, U., Andersson-Cedergren, E., Ottoson, D.: Cellular organization of the frog muscle spindle as revealed by serial sections for electron microscopy. J. Ultrastruct. Res. 14, 1–35 (1966).

  20. Lima-de-Faria, A.: Metabolic DNA in Tipula oleracea. Chromosoma (Berl.) 13, 47–59 (1962).

  21. Lu, B. C.: The course of meiosis and centriole behaviour during the ascus development of the ascomycete Gelasinospera calospera. Chromosoma (Berl.) 22, 210–226 (1967).

  22. Moens, P. B.: A new interpretation of meiotic prophase in Lycopersicon esculentum (tomato). Chromosoma (Berl.) 15, 231–242 (1964).

  23. Moens, P. B.: The fine structure of meiotic chromosome polarization and pairing in Locusta migratoria spermatocytes. Chromosoma (Berl.) 28, 1–25 (1969).

  24. Moens, P. B., Perkins, F. O.: Chromosome number of a small protist: accurate determination. Science 166, 1289–1291 (1969).

  25. Moses, M. J.: Chromosomal structure in crayfish spermatocytes. J. biophys. biochem. Cytol. 2, 215–218 (1956a).

  26. Moses, M. J.: Studies on nuclei using correlated cytochemical, light and electron-microscope techniques. J. biophys. biochem. Cytol. 2, Suppl. 397–406 (1956b).

  27. Moses, M. J.: The relation between the axial complex of meiotic prophase chromosomes and chromosome pairing in a salamander (Plethodon cinereus). J. biophys. biochem. Cytol. 4, 633–638 (1958).

  28. Moses, M. J.: Synaptinemal complex. Ann. Rev. Genet. 2, 363–412 (1968).

  29. Moses, M. J.: Structure and function of the synaptonemal complex. Genetics 61, Suppl. 41–51 (1969).

  30. Payne, F.: The chromosomes of Gryllotalpa borealis. Burm. Sonderabdruck aus dem Arch. Zellforsch. 9, 141–148 (1912).

  31. Payne, F.: A study of the germ cells of Gryllotalpa borealis and Gryllotalpa vulgaris. J. Morph. 28, 287–327 (1916).

  32. Pease, D. C.: Histological techniques for electron microscopy, 2nd ed. New York-London: Academic Press 1964.

  33. Rossen, J. M., Westergaard, M.: Studies on the mechanism of crossing-over. II. Meiosis and the time of meiotic chromosome replication in the ascomycete Neotiella rutilans (Fr.) Dennis. C. R. Lab. Carlsberg 35, 233–260 (1966).

  34. Roth, T. F.: Changes in the synaptinemal complex during meiotic prophase in mosquito oocytes. Protoplasma (Wien) 61, 346–386 (1966).

  35. Schin, K. S.: Meiotische Prophase und Spermatidenreifung bei Gryllus domesticus mit besonderer Berücksichtigung der Chromosomenstruktur. Z. Zellforsch. 65, 481–513 (1965a).

  36. Schin, K. S.: Core-Strukturen in den meiotischen und post-meiotischen Kernen der Spermatogenese von Gryllus domesticus. Chromosoma (Berl.) 16, 436–452 (1965b).

  37. Solari, A. J.: The behaviour of chromosomal axes during diplotene in mouse spermatocytes. Chromosoma (Berl.) 31, 217–230 (1970).

  38. Solari, A. J., Tres, L. L.: The ultrastructure of the human sex vesicle. Chromosoma (Berl.) 22, 16–31 (1967).

  39. Sorokin, S. P.: Reconstructions of centriole formation and ciliogenesis in mammalian lungs. J. Cell Sci. 3, 207–230 (1968).

  40. Sotelo, J. R.: Ultrastructure of the chromosomes at meiosis. In: Handbook of molecular cytology (A. Lima-de-Faría, ed.). Amsterdam: North Holland Research Monographs 1969.

  41. Sotelo, J. R., Trujillo-Cenóz, O.: Electron microscope study of the kinetic apparatus in animal sperm cells. Z. Zellforsch. 48, 565–601 (1958a).

  42. Sotelo, J. R., Trujillo-Cenóz, O.: Electron microscope study on the development of ciliary components of the neural epithelium of the chick embryo. Z. Zellforsch. 49, 1–12 (1958b).

  43. Sotelo, J. R., Wettstein, R.: Fine structure of meiotic chromosomes. Genes and chromosomes — Structure and function. International Symposium. Buenos Aires 1964. Nat. Cancer Inst. Monogr. 18, 133–152 (1965).

  44. Sotelo, J. R., Wettstein, R.: Association of nucleolus and sex chromosomes in Gryllidae spermatocytes. The nucleolus its structure and function. International Symposium. Montevideo, 1965. Nat. Cancer Inst. Monogr. 23, 77–89 (1966).

  45. Sotelo, J. R., Wettstein, R., García, R. B.: Inherited sub-structural arrays in meiotic nuclei of Grylloidea. Rev. Microsc. Electr. 1, 140–141 (1972).

  46. Stockert, J. C., Giménez-Martin, G., Sogo, J. M.: Nucleolus and synaptonemal complexes in pachytene meiocytes of Allium cepa. Cytobiologie (Stuttgart) 2, 235–250 (1970).

  47. Swanson, C. P.: Cytology and cytogenetics. Englewood Cliffs, N. J.: Prentice Hall 1958.

  48. Tosi, M.: The chromosome sets of Gryllotalpa gryllotalpa L. and their geographical distribution. Caryologia (Firenze) 12, 189–198 (1959).

  49. Trujillo-Cenóz, O.: The structural organization of the compound eye in insects. In: Handbook of sensory physiology, vol. VII/2. Berlin-Heidelberg-New York: Springer 1972.

  50. Westergaard, M., Wettstein, D. von: The synaptonemal complex. Ann. Rev. Genet. 6, 71–110 (1972).

  51. Wettstein, R., Sotelo, J. R.: Electron microscope serial reconstructions of the spermatocyte I nuclei at pachytene. J. Microscop. 6, 557–576 (1967).

  52. Wettstein, R., Sotelo, J. R.: The molecular architecture of synaptonemal complexes. Advanc. cell. molec. Biol. 1, 109–152 (1971).

  53. White, M. J. D.: Cytogenetics of orthopteroid insects. Advanc. Genet. 4, 267–330 (1951).

  54. Wilson, E. B.: The cell in development and heredity, 3d edit. New York: The MacMillan Co. (1925).

  55. Winiwarter, H. de.: Etude du cycle chromosomique chez diverses races de Gryllotalpa gryllotalpa (L.). Arch. de Biol. 37, 515–572 (1927).

  56. Wolfe, S. L., John, B.: The organization and ultrastructure of male meiotic chromosomes in Oncopeltus fasciatus. Chromosoma (Berl.) 17, 85–103 (1965).

  57. Wolfe, S. L., John, B., Hewitt, G. M.: The strandedness of meiotic chromosomes from Oncopeltus. J. Cell Biol. 31, 31–42 (1966).

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Sotelo, J.R., García, R.B. & Wettstein, R. Serial sectioning study of some meiotic stages in Scaptericus borrelli (Grylloidea) . Chromosoma 42, 307–333 (1973). https://doi.org/10.1007/BF00284777

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Keywords

  • Microscope Study
  • Serial Section
  • Granular Material
  • Electron Microscope Study
  • Section Study