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Beeinflussung der quantitativen Cytoarchitektur der Rattenleberzelle durch das Cytostaticum Bleomycin

Bleomycine induced changes in the quantitative cytoarchitecture of rat hepatocytes

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Following 3 days of bleomycine administration (2.5 mg/100g body weigth), the following morphometric changes in Wistar rat hepatocytes were determined:

  1. 1.

    The rough endoplasmic reticulum showed partially dilated and partially vesiculated sectors. There was a 33% decrease of both hepatocytic compartimental volume and membrane surface area. Hence, bleomycine has directly impaired the synthesizing efficiency of the liver cells.

  2. 2.

    The free i.e. non-membrane attached ribosomes assume a considerably larger proportion of the cytoplasmic unit volume; they are frequently arranged in semi-circular and spiral-shapped polysomes. This latter finding may be interpreted as structural and functional adaptive reactions.

  3. 3.

    A quantitative reshuffling of hepatocytic chondriomes has been induced by a rather small number of enlarged mitochondria. There has, however, been no change in the volume fraction of the cytoplasmic unit volume. Therefore, an adaptive reaction has also transpired in the energetic-active compartiment.


Nach 3 tägiger Bleomycinverabreichung (je 2,5 mg/100 g Körpergewicht) wurden mit Hilfe der Morphometrie an der Leberparenchymzelle von Wistarratten folgende Veränderungen festgestellt:

  1. 1.

    Das rauhe endoplasmatische Reticulum weist teilweise dilatierte und teilweise vesiculierte Abschnitte auf und büßt ein Drittel sowohl seines Kompartimentvolumens als auch seiner Membranoberfläche ein. Demzufolge beeinträchtigt Bleomycin die hepatocelluläre Syntheseleistung.

  2. 2.

    Die freien (d. h. nicht membrangebundenen) Ribosomen nehmen im Einheitsvolumen Cytoplasma beträchtlich zu und sind häufig zu semizirkulären und spiralförmigen Polysemen angeordnet, was als Ausdruck einer strukturellen und funktionellen Anpassungsreaktion gedeutet wird.

  3. 3.

    Das hepatocelluläre Chondriom wird durch eine kleinere Anzahl vergrößerter Mitochondrion ohne Veränderung ihres Volumenanteils am Einheitsvolumen Cytoplasma quantitativ umgegliedert, so daß auch im energetisch aktiven Kompartiment eine Anpassungsreaktion erfolgt.

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Riede, U.N., Kaiser, W., von Matt, C. et al. Beeinflussung der quantitativen Cytoarchitektur der Rattenleberzelle durch das Cytostaticum Bleomycin. Z. Krebsforsch. 80, 323–334 (1973).

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