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Quantitative cytochemical investigations on Ehrlich ascites tumour cells treated with Vincristine

II. Fast green cytophotometric investigations

Summary

Changes in the basic protein content of Ehrlich ascites tumour cells in mice treated with 2 mg/kg Oncovin (Lilly) and 2 mg/kg Vincristine (ssVCR) (Richter), respectively were studied by Fast green Cytophotometric method during a 25-day period following treatment.

The amount of cellular basic proteins rose considerably already on the 2nd day after treatment. On the 3rd day further increase could be observed then up to the 10th day this elevated histone content was characteristic of the cells. A slight decrease was noted on the 11th, 14th, 18th and 25th days which, however, always remained considerably higher than in the control.

No difference between the effect of the two Vincristine preparations on the cellular basic protein content could be demonstrated.

Zusammenfassung

Die Veränderung des basischen Proteingehalts der mit 2 mg/kg Oncovin (Lilly) oder mit 2 mg/kg Vincristin (Richter) behandelten Ehrlichschen Mäuse-Ascitestumorzellen wurde im Verlauf von 25 Tagen nach der Behandlung mit der cytophotometrischen Fastgreen-Methode untersucht.

Bereits am zweiten Tage nach der Behandlung zeigte die Menge der cellulären basischen Proteine einen bedeutenden Anstieg. Am dritten Tage ließ sich ein weiterer Anstieg wahrnehmen, und anschließend war bis zum 10. Tage für die Zellen im wesentlichen dieser gesteigerte Histongehalt kennzeichnend. Am 11., 14., 18. und 25. Tage nahm dieser Wert etwas ab, blieb aber immer noch erheblich höher als in den Kontrollzellen.

In bezug auf die Wirkung der beiden Vincristin-Präparate auf den basischen Proteingehalt der Zellen ließ sich kein Unterschied aufzeigen.

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Gyeskó, A., Szentirmay, Z. & Sugár, J. Quantitative cytochemical investigations on Ehrlich ascites tumour cells treated with Vincristine. Z. Krebsforsch. 78, 90–98 (1972). https://doi.org/10.1007/BF00284317

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Keywords

  • Public Health
  • Cancer Research
  • Vincristine
  • Basic Protein
  • Fast Green