The chromosomal complement of the human cell line D98/AH-2 has been studied by quinacrine mustard and trypsin Giemsa banding techniques. The dispersion of chromosome counts has been shown to be due to non-random variation involving mainly a few particular chromosomes. — Twelve different marker chromosomes could be distinguished and the presumptive derivation of most of their chromosomal material from normal human chromosomes has been determined. Most cells in 6 different hybrid clones derived from fusion of D98/AH-2 cells with skin fibroblasts from a cystinotic patient contained a single copy of each marker chromosome.
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Supported by: united States Public Health Service Grant HD 04608, National Institute of General Medical Sciences Grant GM 17702 and American Heart Association Grant 71-981.
Established Investigator of the American Heart Association.
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Francke, U., Hammond, D.S. & Schneider, J.A. The band patterns of twelve D 98/AH-2 marker chromosomes and their use for identification of intraspecific cell hybrids. Chromosoma 41, 111–121 (1973). https://doi.org/10.1007/BF00284079
- Band Pattern
- Human Chromosome
- Skin Fibroblast
- Human Cell Line