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Molecular and General Genetics MGG

, Volume 243, Issue 1, pp 32–38 | Cite as

Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants

  • Frank T. Röder
  • Thomas Schmülling
  • Christiane Gatz
Original Paper

Abstract

We have investigated the use of the tetracycline-dependent gene expression system to regenerate and propagate tobacco plants transformed with a gene whose product — when highly expressed — interferes with regeneration and/or further reproduction. Plants transformed with the Agrobacterium rhizogenes rolB gene under the control of the tetracycline-dependent expression system were phenotypically indistinguishable from wild type owing to efficient repression of the promoter. Induction of the rolB gene with tetracycline led to high-level expression of the rolB mRNA, which resulted in extremely stunted plants with necrotic and wrinkled leaves that did not develop a floral meristem. Upon cessation of tetracycline treatment healthy shoots developed even from severely affected meristems. Data on the dose response of the rolB phenotype as a function of tetracycline concentration demonstrate that the tetracycline-dependent gene expression system can be used to modulate the manifestation of a particular phenotype.

Key words

Regulated expression rolB gene Tetracycline Tet repressor 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Frank T. Röder
    • 1
  • Thomas Schmülling
    • 1
  • Christiane Gatz
    • 1
  1. 1.Institut für Genbiologische Forschung Berlin GmbHBerlinGermany

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