Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis:

1. Detection of genetic variant polypeptides in PHA-stimulated peripheral blood lymphocytes

  • 28 Accesses

  • 21 Citations


Approximately 250 phytohemagglutinin (PHA)-stimulated peripheral blood lymphocyte polypeptides from three unrelated healthy males were compared by high-resolution two-dimensional gel electrophoresis and double-label autoradiography. Comparisons by all possible pairwise combinations of [14C]leucine-labeled proteins from an individual and [3H]leucine-labeled proteins from another revealed that only three polypeptides differed qualitatively among the three individuals. The degree of variation in lymphocyte polypeptides between different individuals was similar to that in fibroblast polypeptides reported previously. Among the three variant polypeptides, two polypeptides with mol.wt. 64,000 and mol. wt. 37,000 coexisted with a polypeptide with the same molecular weight, and they showed the behavior expected of two allelic gene products separated in the isoelectric focusing dimension by charge differences. Analysis of [14C]leucine labeled peripheral blood lymphocyte proteints, from the parents of each individual, by two-dimensional gel electrophoresis indicated that the variant polypeptides with mol. wt. 64,000 and mol. wt. 37,000 in the propositus were inherited from one of his parents. The data indicate that genetic analysis of PHA-stimulated peripheral blood lymphocyte proteins is feasible by high-resolution two-dimensional gel electrophoresis in combination with double-label autoradiography and pedigree analysis.

This is a preview of subscription content, log in to check access.


  1. Giblett ER (1977) Genetic polymoprhisms in human blood. Annu Rev Genet 11:13–28

  2. Harris H, Hopkinson DA, Edwards YH (1977) Polymorphism and the subunit structure of enzymes: A contribution to the neutralist-selectionist controversy. Proc Natl Acad Sci USA 74:698–701

  3. Lester EP, Lemkin P, Lipkin L, Cooper HL (1981) A two-dimensional electrophoretic analysis of protein synthesis in resting and growing lymphocytes in vitro. J Immunol 126:1428–1434

  4. McConkey EH (1979) Double-label autoradiography for comparison of complex protein mixtures after gel electrophoresis. Anal Biochem 96:39–44

  5. McConkey EH, Taylor BJ, Phan D (1979) Human heterozygosity: A new estimate. Proc Natl Acad Sci USA 76:6500–6504

  6. O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

  7. Walton KE, Styer D, Gruenstein EI (1979) Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem 254:7951–7960

Download references

Author information

Correspondence to Hideo Hamaguchi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hamaguchi, H., Ohta, A., Mukai, R. et al. Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis:. Hum Genet 59, 215–220 (1981). https://doi.org/10.1007/BF00283667

Download citation


  • Molecular Weight
  • Polypeptide
  • Genetic Analysis
  • Gene Product
  • Leucine