Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The Mu1 transposable element of maize contains two promoter signals recognized by the Escherichia coli RNA polymerase

  • 41 Accesses

  • 2 Citations

Summary

The galactokinase (GaIK) expression plasmid vector system pK0-1 has been used to screen for promoter elements in the maize transposable element Mu1 that function in Escherichia coli. Two transcriptional start points, named S1 and S2, were identified, which are located in the two direct repeats of the transposable element. This paper demonstrates that sequence elements exist in a plant transposable element which function as prokaryotic promotors.

This is a preview of subscription content, log in to check access.

References

  1. Barker RF, Thompson DV, Talbot DR, Swanson JA, Bennetzen JL (1984) Nucleotide sequence of the maize transposable element Mu1. Nucleic Acids Res 12:5955–5967

  2. Bennetzen JL (1984) Transposable element Mu1 is found in multiple copies only in Robertson's Mutator maize lines. J Mol Appl Genet 2:519–524

  3. Bennetzen JL, Swanson J, Taylor WC, Freeling M (1984) DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci USA 81:4125–4128

  4. Bennetzen JL, Fracasso RP, Morris DW, Robertson DS, SkogenHagenson MJ (1987) Concomitant regulation of Mul transposition and mutator activity in maize. Mol Gen Genet 208:57–62

  5. Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523

  6. Boyer HW, Roulland-Dussoin D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472

  7. Broyles SS, Moss B (1986) Homology between RNA polymerase of poxviruses, prokaryotes and eukaryotes: nucleotide sequence and transcriptional analysis of Vaccinia virus genes encoding 147-KDa and 22-KDa subunits. Proc Natl Acad Sci USA 83:3141–3145

  8. Bukhari AI (1976) Bacteriophage Mu as a transposition element. Annu Rev Genet 10:389–412

  9. Chandler VL, Rivin CJ, Walbot V (1986) Stable non-mutator lines of maize have elements homologous to the Mu1 transposable element. Genetics 114:1007–1021

  10. Clewell DB, Helinski DR (1969) Supercoiled circular DNA-protein complex in E. coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166

  11. Del Giudice L (1979) Method for isolating restriction and modificationless mutants of Escherichia coli K12. J Bacteriol 137:673–676

  12. Del Giudice L (1981) Cloning of mitochondrial DNA from the petite-negative yeast Schizosaccharomyces pombe in the bacterial plasmid pBR322. Mol Gen Genet 184:465–470

  13. Del Giudice L, Manna F, Massardo DR, Wolf K, Motto M (1987) Cryptic copies of Mu1 transposon in Italian maize varieties. Maydica 32:189–200

  14. Favoloro J, Treisman R, Kamen R (1980) Transcription maps of polyoma virus specific RNA: Analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol 65:718–749

  15. Ghosal D, Saedler H (1978) DNA sequence of the mini-insertion IS2-6 and its relation to the sequence of IS2. Nature 275:611–617

  16. Gill R, Hefron F, Dougan G, Falkow S (1978) Analysis of sequences transposed by complementation of 2 classes of transposition deficient mutants of transposition element Tn3. J Bacteriol 136:742–756

  17. Goldberg DA (1980) Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc Natl Acad Sci USA 77:5794–5798

  18. Grisolia V, Carlomagno S, Bruni CB (1982) Cloning and expression of the distal portion of the histidine operon of Escherichia coli K-12. J Bacteriol 151:692–700

  19. Hollenberg CP (1980) Recombinant DNA research. An update of techniques and results. Prog Bot 42:171–185

  20. Kleckner N (1981) Transposable elements in prokaryotes. Annu. Rev Genet 15:341–404

  21. Kong XF, Lovett PS, Kung SD (1984) The Nicotiana chloroplast genome. IX. Identification of regions active as prokaryotic promoters in Escherichia coli. Gene 31:23–30

  22. Kwak JW, Kim J, Yoo OJ, Han MM (1987) A method to isolate DNA sequences that are promoter-active in Escherichia coli and in yeast. Biochem Biophys Res Commun 81:636–643

  23. Lillis M, Freeling M (1986) Mu transposon in maize. Trends Genet 2:183–188

  24. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor, Laboratory, Cold Spring Harbor, New York

  25. Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavage. Methods Enzymol 65:499–560

  26. McKenney K, Shimatake H, Court D, Schmeissner U, Brady C, Rosenberg M (1981) A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. In: Chirikjian JG, Papas TS (eds) Gene amplification and analysis, vol 2. Structural analysis of nucleic acids. Elsevier, New York, pp 282–415

  27. Neve RL, West RW, Rodriguez RL (1979) Eukaryotic DNA fragments which act as promoters for a plasmid gene. Nature 277:324–325

  28. Nevers P, Shepherd NS, Saedler H (1985) Plant transposable elements. Adv Bot Res 12:102–203

  29. Prentki P, Teter B, Chandler M, Galas DJ (1986) Functional promoters created by the insertion of transposable element IS1. J Mol Biol 191:282–292

  30. Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase 1. J Mol Biol 113:237–251

  31. Robertson DS (1978) Characterization of mutator system in maize. Mutat Res 51:21–28

  32. Robertson DS, Stinard PS (1987) Genetic evidence of mutatorinduced deletion in the short arm of chromosome 9 of maize. Genetics 115:353–361

  33. Rosenberg M, Chepelinsky AB, McKenney K (1983) Studying promoters and terminators by gene fusion. Science 222:734–739

  34. Strommer JM, Hake S, Bennetzen J, Taylor WC, Freeling M (1982) Regulatory mutants of the maize Adhl gene caused by DNA insertions. Nature 300:542–544

  35. Sweetser D, Nonet M, Young RA (1987) Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Proc Natl Acad Sci USA 84:1192–1196

  36. Taylor LP, Walbot V (1987) Isolation and characterization of a 1.7-kb transposable element from a mutator line of maize. Genetics 117:297–307

  37. Taylor LP, Chandler VL, Walbot V (1986) Insertion of 1.4 kb and 1.7 kb Mu elements into the Bronze 1 gene of Zea mays. Maydica 31:31–45

  38. Thomas PS (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natt Acad Sci USA 77:5201–5205

  39. Vayda ME, Freeling M (1986) Insertion of the Mu1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure. Plant Mol Biol 6:441–454

  40. Zuker C, Cappello J, Chisholm RL, Lodish HF (1983) A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock. Celt 34:997–1005

  41. Zuker C, Cappello J, Lodish HE, George P, Chung S (1984) Dic-tyostelium transposable element DIRS-1 has 350-base-pair inverted terminal repeats that contain a heat shock promoter. Proc Natl Acad Sci USA 81:2660–2664

Download references

Author information

Correspondence to Luigi Del Giudice.

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Del Giudice, L., Manna, F., Massardo, D.R. et al. The Mu1 transposable element of maize contains two promoter signals recognized by the Escherichia coli RNA polymerase. Molec. Gen. Genet. 222, 71–76 (1990). https://doi.org/10.1007/BF00283025

Download citation

Key words

  • Transposable elements
  • Promoters
  • Maize
  • Escherichia coli