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Aspergillus nidulans nuclear proteins bind to a CCAAT element and the adjacent upstream sequence in the promoter region of the starch-inducible Taka-amylase A gene


Aspergillus nidulans was used as an intermediate host to investigate the regulation of the Takaamylase A (TAA) gene from Aspergillus oryzae. The induction of Taa by starch was confirmed to be regulated at the transcriptional level by analyzing the transcripts specific for Taa synthesized in vitro in nuclei from starch-and glucose-grown cells. A 55 by DNA fragment containing a consensus CCAAT sequence from the promoter region of the Taa gene was shown to confer starch inducibility on the gene. A nuclear extract from starch-grown cells was assayed for proteins which bind to the promoter region of the Taa gene. A protein designated AnCP1 bound to the CCAAT sequence. A nuclear extract from glucose-grown cells contained two DNA-binding proteins designated AnCP2 and AnNP1. AnCP2 bound to the same CCAAT sequence as AnCPI, while AnNPI bound to the 25 by region just upstream of the AnCP2 binding site. Occupancy of the two binding sites appeared to be mutually exclusive, which is suggestive of a negative regulatory mechanism for gene expression.

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Correspondence to Norihiro Tsukagoshi.

Additional information

Communicated by C.A.M.J.J. van den Hondel

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Nagata, O., Takashima, T., Tanaka, M. et al. Aspergillus nidulans nuclear proteins bind to a CCAAT element and the adjacent upstream sequence in the promoter region of the starch-inducible Taka-amylase A gene. Molec. Gen. Genet. 237, 251–260 (1993).

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Key words

  • Aspergillus nidulans
  • Taka-amylase A
  • DNA binding proteins
  • AnCP1, AnCP2, AnNPI
  • Negative regulatory mechanism