Advertisement

Theoretical and Applied Genetics

, Volume 40, Issue 4, pp 173–175 | Cite as

The effect of cycloheximide and 6-methylpurine on in vivo compatible and incompatible pollen tube growth in Lilium longiflorum

  • Peter D. Ascher
  • Lyndon W. Drewlow
Article

Summary

Cycloheximide, a potent inhibitor of protein synthesis, placed in styles of Lilium longiflorum at 10−4 M in stigmatic exudate before, 6, or 12 hr after compatible or incompatible pollination retarded all pollen tube growth. An inhibitor of RNA synthesis, 6-methylpurine, placed in the style at 10−4 M in stigmatic exudate before, 6, or 12 hr after pollination restricted compatible pollen tube growth to lengths not significantly different thanincompatiblepollen tubes in treated or nontreated styles. While pollen tube growth in the style of L. longiflorum appears to require protein synthesis, only compatible pollen tube growth requires RNA synthesis. Stigmatic exudate proved to be an excellent carrier of exogenous substances into the style of L. longiflorum.

Keywords

Protein Synthesis Pollen Tube Potent Inhibitor Cycloheximide Tube Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ascher, P. D.: A gene action model to explain gametophytic self-incompatibility. Euphytica 15, 179 to 183 (1966).Google Scholar
  2. 2.
    Ascher, P. D., Drewlow, L. W.: Effect of stigmatic exudate injected into the stylar canal on compatible and incompatible pollen tube growth in Lilium longiflorum Thunb. International Conference on Pollen Physiology, Pullman Wash. (1969).Google Scholar
  3. 3.
    Ascher, P. D., Peloquin, S. J.: Pollen tube growth and incompatibility following intra and interspecific pollinations in Lilium longiflorum. Amer. J. Bot. 55, 1230–1234 (1968).Google Scholar
  4. 4.
    Crang, R. E., Miles, G. B.: An electron microscope study of germinating Lychnis alba pollen. Amer. J. Bot. 56, 398–405 (1969).Google Scholar
  5. 5.
    Ennis, H. L., Lubin, M.: Cycloheximide and acetoxycycloheximide: Inhibitors of transfer of amino acid from aminoacyl-s-RNA to polypeptide in mammalian cells. Fed. Proc. 23, 269 (1964).Google Scholar
  6. 6.
    Jensen, W. A., Fisher, D. B., Ashton, M. E.: Cotton embryogenesis: The pollen cytoplasm. Planta 81, 206–228 (1968).Google Scholar
  7. 7.
    Lin, S. Y., Mosteller, R. D., Hardesty, B.: The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell free reticulocyte system. J. Mol. Biol. 21, 51–69 (1966).Google Scholar
  8. 8.
    Linskens, H. F.: Physiologische Untersuchungen der Pollenschlauch-Hemmung selbststeriler Petunien. Z. Bot. 43, 1–44 (1955).Google Scholar
  9. 9.
    Linskens, H. F.: Isolation of ribosomes from pollen. Planta 73, 194–200 (1967).Google Scholar
  10. 10.
    Linskens, H. F., Tupý, J.: The amino acids pool in the style of self-incompatible strains of Petunia after self and cross-pollination. Der Züchter 36, 151–158 (1966).Google Scholar
  11. 11.
    Mascarenhas, J. P.: Pollen tube growth and ribonucleic acid synthesis by vegetative and generative nuclei of Tradescantia. Amer. J. Bot. 53, 563–569 (1966).Google Scholar
  12. 12.
    Mascarenhas, J. P., Bell, E.: Protein synthesis during germination of pollen, studies on polyribosome formation. Biochim. Biophys. Acta 179, 199–203 (1969).Google Scholar
  13. 13.
    Miller, J. H., Kempner, E. S.: Effects of an adenine analog on yeast metabolism. Biochim. Biophys. Acta 76, 333–340 (1963).Google Scholar
  14. 14.
    Siegel, M. R., Sisler, H. D.: Site of action of cycloheximide in cells of Saccharomyces pastorianus II. The nature of inhibition of protein synthesis in a cell free system. Biochim. Biophys. Acta 87, 83–89 (1964).Google Scholar
  15. 15.
    Steffensen, D. M.: Synthesis of ribosomal RNA during growth and division in Lilium. Exp. Cell Res. 44, 1–12 (1966).Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Peter D. Ascher
    • 1
  • Lyndon W. Drewlow
    • 1
  1. 1.Department of Horticultural ScienceUniversity of MinnesotaSt. PaulUSA

Personalised recommendations