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Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig

Summary

In this study we extracted guinea pig brain and testis with; the extract was adsorbed to and eluted from cartridges (the Sep-Pak C 18 procedure). We found this procedure superior for recovering crystalline insulin added to buffers or tissues, and for recovering endogenous insulin from plasma, but inferior for recovery of insulin from tissues. However, we did find ‘rat/pork’ type-insulin in guinea pig brain and testis (5–50 pg/g wet weight tissue). Our results with the Sep-Pak C18 procedures were reproduced by four other laboratories (who found 4–60 pg/g wet weight of tissue) and similar findings were also obtained by an independent investigator. Thus, we conclude that extrapancreatic tissues of guinea pigs have a second type of insulin-related material that is more typical of other mammalian insulins, but that the amount recovered is dependent upon the extraction procedure utilized.

References

  1. 1.

    Rosenzweig JL, Lesniak MA, Samuels BE, Yip CC, Zimmerman AE, Roth J (1980) Insulin in the extrapancreatic tissues of guinea pigs differs markedly from the insulin in their pancreas and plasma. Trans Assoc Am Physicians 93: 263–278

  2. 2.

    Eng J, Yalow RS (1982) Absence of pork-like insulin in guinea pig tissues. Proc Natl Acad Sci USA 79: 2683–2685

  3. 3.

    Stevenson RW (1983) Further evidence for non-pancreatic insulin immunoreactivity in guinea pig brain. Horm Metab Res 15: 526–528

  4. 4.

    Havrankova J, Roth J, Brownstein MJ (1979) Concentrations of insulin and of insulin receptors in the brain are independent of peripheral insulin levels. J Clin Invest 64: 636–642

  5. 5.

    Eng J, Yalow RS (1981) Evidence against extrapancreatic insulin synthesis. Proc Natl Acad Sci USA 78: 4576–4578

  6. 6.

    LeRoith D, Shiloach J, Roth J, Lesniak MA (1980) Evolutionary origins of vertebrate hormones, substances similar to mammalian insulins are native to unicellular eukaryotes. Proc Natl Acad Sci 77: 6184–6188

  7. 7.

    Zimmerman AE, Yip CC (1974) Guinea pig insulin I: purification and physical properties. J Biol Chem 249: 4021–4025

  8. 8.

    Zimmerman AE, Moule ML, Yip CC (1974) Guinea pig insulin II: biological activity. J Biol Chem 249: 4026–4030

  9. 9.

    LeRoith D, Hendricks SA, Lesniak MA, Rishi S, Becker KL, Havrankova J, Rosenzweig J, Brownstein MJ, Roth J (1983) Insulin and other extrapancreatic tissues of vertebrates and non-vertebrates. In: Szabo AJ (ed) Advances in metabolic disorders: CNS regulation of carohydrate metabolism, Vol.10. Academic Press, New York, pp. 304–340

  10. 10.

    Eng J, Yalow RS (1980) Insulin recoverable from tissues. Diabetes 29: 105–109

  11. 11.

    Rosenzweig JL, LeRoith D, Lesniak MA, MacIntyre I, Sawyer WH, Roth J (1983) Two distinct insulins in the guinea pig: the broad relevance of these findings to evolution of peptide hormones. Fed Proc 42: 2608–2614

  12. 12.

    Chan SJ, Episkopov V, Zeitlin S, Karathanasis SD, Mackrell A, Steiner DF, Efstratiadis A (1984) Proc Natl Acad Sci 81: 5046–5050

  13. 13.

    Muglia L, Locker J (1984) Extrapancreatic insulin gene expression in the fetal rat. Proc Natl Acad Sci 81: 3635–3639

  14. 14.

    Birch NP, Christie DL, Renwick AGC (1984) Proinsulin-like material in mouse fetal brain cell cultures. FEBS Lett 168: 299–302

  15. 15.

    Birch NP, Christie DL, Renwick AGC (1984) Immunoreactive insulin from mouse brain cells in culture and whole rat brain. Biochem J 218: 19–27

  16. 16.

    Budd GC, Pansky B, Cordell B (1983) Insulin or insulin-like peptides in the pituitary gland. J Cell Biol 97: 404A

  17. 17.

    Lee CH, Lin BC, Costa E, Chuang DM (1984) Detection of mRNA species in the brain and other tissues of rat which hybridize with proinsulin gene. Fed Proc 43: 2219 (Abstract)

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Rosenzweig, J.L., LeRoith, D., Lesniak, M.A. et al. Two distinct insulin-related molecules in the guinea pig: immunological and biochemical characterization of insulin-like immunoactivity from extrapancreatic tissues of the guinea pig. Diabetologia 28, 237–243 (1985). https://doi.org/10.1007/BF00282240

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Key words

  • Guinea pig
  • extrapancreatic tissues
  • insulin-related immunoactivity