In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
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This study will be extended to other developmental stages and to different organs. For instance, on 2D gels obtained from 3 day-old etiolated shoots we noticed many differences from the 8 day-old leaf studied here (Zivy et al., in preparation). The way we read, analyse and compare our gels by looking at the spots one by one on the 36 gels is a long and eye-tiring method. Moreover it only allows a very rough quantitative approach. We are presently developing a reading system of the gel image based on a video camera and a microcomputer. The comparative analysis will be performed with the use of programs developed by Anderson's laboratory at the Argonne National Laboratory (Anderson et al. 1981). It is very important to obtain reliable quantitative estimations of spots since, according to Klose (1982), many genetical differences can be noticed at the level of protein concentration, i.e. at the regulation level.
Communicated by K. Tsunewaki
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Zivy, M., Thiellement, H., de Vienne, D. et al. Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis. Theoret. Appl. Genetics 66, 1–7 (1983). https://doi.org/10.1007/BF00281838
- Triticum aestivum
- Aegilops species
- Alloplasmic lines
- 2D gel electrophoresis
- Cytoplasmic inheritance