Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Pollen protein synthesis and control of incompatibility in Brassica

  • 43 Accesses

  • 11 Citations

Summary

Excised but otherwise intact cabbage (Brassica oleracea var. capitata) stigmas release a watersoluble substance which selectively inhibits germination of self-but not cross-pollen. The inhibitory effect on self-pollen germination is dependent on both the concentration of stigma extract and on the time of addition. Low concentrations of stigma extract inhibit when present from the start (zero time additions) of pollen imbibition, whereas high concentrations do not. High concentrations inhibit when stigma extracts are added 1 to 2 minutes after the start of pollen imbibition, but germination is increasingly less inhibited when additions are delayed 2 to 4 minutes. Similar inhibition kinetics are also observed with delayed additions of cordycepin and cycloheximide. Stigma extracts selectively inhibit leucine-14C incorporation into proteins of self-pollen. We conclude that germination does not require protein synthesis whereas the regulation of self-incompatibility does.

This is a preview of subscription content, log in to check access.

Literature

  1. Arasu, N.T.: Self-incompatibility in angiosperms: a review. Genetica 39, 1–24 (1968)

  2. Ascher, P.D.; Drewlow, L.W.: The effect of cycloheximide and 6-methyl purine on in vivo compatible and incompatible pollen tube growth in Lilium langiflorum. Theoret. Appl. Genetics 40, 173–175 (1970)

  3. Ferrari, T.E.; Wallace, D.H.: Germination of Brassica pollen and expression of incompatibility in vitro. Euphytica 24, 757–765 (1975)

  4. Ferrari, T.E.; Widholm, J.M.: A simple, rapid, and sensitive method for estimation of DNA, RNA, and protein synthesis in carrot cell cultures. Analyt. Biochem. 56, 346–352 (1973)

  5. Heslop-Harrison, J.: Incompatibility and the pollenstigma interaction. Ann. Rev. Plant Physiol. 26, 403–425 (1975)

  6. Heslop-Harrison, J. ; Knox, R.B.; Heslop-Harrison, Y.: Pollen-wall proteins: exine-held fractions associated with the incompatibility response in Cruciferae. Theoret. Appl. Genetics 44, 133–137 (1974)

  7. Kroh, M.: Reaction of pollen after transfer from one stigma to another (Contribution to the character of the incompatibility mechanism in Cruciferae). Züchter 36, 185–189 (1967)

  8. Mascarenhas, J.P.: The biochemistry of angiosperm pollen development. Bot. Rev. 41, 259–314(1975)

  9. Murabaa, A.I.M.et: Factors affecting seed set in Brussels sprouts, radish and cyclamen. Publ. 168 Lab. voor Tuinbouwplantenteelt Landbouwhogeschool, Wageningen, Nederland (1957)

  10. Nasrallah, M.E.: Genetic control of quantitative variation in self-incompatibility proteins detected by immunodiffusion. Genetics 76, 45–50 (1974)

  11. Nasrallah, M.E.; Barber, J.T. ; Wallace, D.H.: Self-incompatibility proteins in plants: detection, genetics, and possible mode of action. Heredity 25, 23–27 (1970)

  12. Nasrallah, M.E.; Wallace, D.H.: Immunogenetics of self-incompatibility in Brassica oleracea L. Heredity 22, 519–527 (1967)

  13. Nasrallah, M.E. ; Wallace, D.H.: The influence of modifier genes on the intensity and stability of self-incompatibility in cabbage. Euphytica 17, 495–503 (1968)

  14. Ockendon, D.J.: Dominance relationships between S-alleles in the stigmas of Brussels sprouts (Brassica oleracea var. gemmifera.) Euphytica 24, 165–172 (1975)

  15. Sedgley, M.: The concentration of S-protein in stigmas of Brassica oleracea plants homozygous and heterozygous for a given S-allele. Heredity 33, 412–416 (1974)

  16. Shivanna, K.R.; Jaiswal, V.S.; Mohan Ram, H.Y.: Inhibition of gamete formation by cycloheximide in pollen tubes of Impatiens balsamina. Planta (Berl.) 117, 173–177 (1974a)

  17. Shivanna, K.R.; Jaiswal, V.S. ; Mohan Ram, H.Y.: Effect of cycloheximide on cultured pollen grains of Trigonella foenumgraecum. Plant Science Letters 3, 335–339 (1974b)

  18. Thompson, K.F.; Taylor, J.P.: Non-linear dominance relationships between S alleles. Heredity 21, 345–362 (1966)

  19. Thompson, K.F.: Classified S alleles for Brassica breeders. In: Dixon, G.G. (Ed.), Proceedings of Brassica Meeting of Eucarpia, pp. 25–28. National Vegetable Research Station, Wellesbourne, England (1968)

Download references

Author information

Additional information

Department of Vegetable Crops Publication No. VC 718.

Communicated by H.F. Linskens

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ferrari, T.E., Wallace, D.H. Pollen protein synthesis and control of incompatibility in Brassica . Theoret. Appl. Genetics 48, 243–249 (1976). https://doi.org/10.1007/BF00281287

Download citation

Keywords

  • Protein Synthesis
  • Cycloheximide
  • Time Addition
  • Brassica Oleracea
  • Cordycepin