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Histochemie

, Volume 18, Issue 2, pp 168–173 | Cite as

Histochemical studies on the lipids in the epithelium of the fetal colon

  • Ch. Garbarsch
Article

Summary

Large intestines from 30 fetuses with crown-rump lengths ranging from 26 to 180 mm have been studied histochemically as to their epithelial content of lipids. These lipids have been localized intracellularly and basally in the cells, and comparative studies of the colon and the small intestine have demonstrated that there are considerable amounts of lipids in the colon as compared with the small intestine.

It is concluded that the main part of the lipids consist of saturated and unsaturated constituents.

The possible explanations of the lipids as absorbed material, biological active material synthetized de novo or as material involved in the metabolism of fetal surface cells are discussed.

Keywords

Public Health Lipid Surface Cell Small Intestine Main Part 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barka, T., and P. J. Anderson: Histochemistry: Theory, practice and bibliography. New York: Harper & Row Inc. 1965.Google Scholar
  2. Bergström, S., L. A. Carlson, and J. R. Weeks: The prostaglandins: A family of biologically active lipids. Pharmacol. Rev. 20, 1–48 (1968).Google Scholar
  3. Chu, C. H. U.: A histochemical study of staining the axis cylinder with fuchsin-sulphorous acid (Schiffs reagent). Anat. Rec. 108, 723–745 (1950).Google Scholar
  4. Dunnigan, M. G.: The use of Nile blue sulphate in the histochemical identification of phospholipids. Stain Technol. 43, 249–256 (1968).Google Scholar
  5. Garbarsch, Ch., and F. Bülow: To be published.Google Scholar
  6. Hartmann, J. F., and L. J. Wells: Fate of food introduced directly into the fetal stomach. Proc. Soc. exp. Biol. Med. (N.Y.) 68, 327–330 (1948).Google Scholar
  7. Hori, S. H.: A simplified acid hematein test for phospholipids. Stain Technol. 38, 221–225 (1963).Google Scholar
  8. Hoyes, A. D.: Electron microscopy of the surface layer (periderm) of the human foetal skin. J. Anat. (Lond.) 103, 321–336 (1968).Google Scholar
  9. Kasten, F. H.: The chemistry of Schiffs reagent. Int. Rev. Cytol. 10, 1–100 (1960).Google Scholar
  10. Liang, Hsu-Mu.: A new method for staining nerves and their endings using the Schiff reaction. Anat. Rec. 99, 511–521 (1947).Google Scholar
  11. Lillie, R. D.: Histopathologic technic and practical histochemistry, 3rd ed. Place: McGrawHill Book Co. 1965.Google Scholar
  12. Nissen, H. M.: On lipid droplets in renal interstitial cells. I. A histochemical study. Z. Zellforsch. 83, 76–81 (1967).Google Scholar
  13. —: On lipid droplets in renal interstitial cells. III. A histological study on the number of droplets during hydration and dehydration. Z. Zellforsch. 92, 52–61 (1968).Google Scholar
  14. Onazaki, T.: Histologische Beiträge zur Frage der Fettresorption aus dem Dickdarm. Tohoku J. exp. Med. 29, 224–243 (1936).Google Scholar
  15. Parvis, V. P., and S. Forni: Studio istochemico della epidermide fetale. Acta histochem. (Jena) 7, 155–174 (1959).Google Scholar
  16. Pearse, A. G. E.: Histochemistry. Theoretical and applied. 1st ed. London: J. & A. Churchill Ltd. 1968.Google Scholar
  17. Pickles, V. R.: The prostaglandins. Biol. Rev. 42, 614–652 (1967).Google Scholar
  18. Pihl, B. G., D. J. Glotzer, and J. F. Patterson: Absorption of the medium-chain fatty acids by the dog colon. J. appl. Physiol. 21, 1059–1062 (1966).Google Scholar
  19. Schmidt, W.: Über den paraplacentaren, fruchtwassergebundenen Stofftransport beim Menschen. II. Nachweis der vom Amnion abgegebenen Lipide im Fruchtwasser und im Dünndarm des Keimes. Z. Anat. Entwickl.-Gesch. 126, 276–288 (1967).Google Scholar
  20. Stickel, M.: Untersuchungen an menschlichen Neugeborenen über das Verhalten des Darmepithels bei verschiedenen funktionellen Zuständen. Arch. Gynäk. 92, 607–658 (1910).Google Scholar
  21. Strauss, E. W.: Morphological aspects of triglyceride absorption. In: Handbook of physiology, vol. III/6, p. 1377–1406 (ed. Ch. F. Code). Amer. Physiological Soc. Washington, D.C., 1968.Google Scholar
  22. Vander, A. J.: Direct effects of prostaglandin on renal function and renin release in anesthetized dog. Amer. J. Physiol. 214, 218–221 (1968).Google Scholar

Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • Ch. Garbarsch
    • 1
  1. 1.Laboratory of Cyto-and Histochemistry, Anatomy Department AUniversity of CopenhagenDenmark

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