In a study of the expression of folate-sensitive fragile sites in five normal individuals using RPMI medium containing methotrexate (MTX), we observed a high frequency of “sister chromatid intercrossings” (SCI) that is, the intersection of sister chromatids. The location of SCIs corresponded to fragile sites in 54.2% of the cases. Of the SCIs observed in each individual, 43%–53% were located at the same bands as their expressed fragile sites. Furthermore when RPMI+MTX medium was used instead of F-10 medium, the incidence of SCIs increased tenfold. We suggest that SCIs could indicate the existence of a pre-lesion.
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Amarose AP, Huttenlocher PR, Sprudzs RM, Laitsch TJ, Pattenati MJ (1987) A heritable fragile 12q24.13 segregating in a family with the fragile X chromosome. Hum Genet 75:4–6
Barbi G, Steinbach P, Vogel W (1984) Non-random distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid sensitive fragile sites. Hum Genet 68: 290–294
Berger R, Bloomfield CD, Sutherland GR (1985) Report of the Commitee on Chromosome Rearrangements in Neoplasia and on Fragile Sites. (8th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 40:490–535
Chapelle A de la, Berger R (1984) Human Gene Mapping 7. Los Angeles Conference (1983). (7th International Workshop on Human Gene Mapping). Cytogenet Cell Genet 37:1–4
Kuhn EM, Therman E (1986) Cytogenetics of Bloom's syndrome. Cancer Genet Cytogenet 22:1–18
Shabtai F, Hart J, Klar D, Halbrecht I (1986a) Familial fragile site found at the cancer breakpoint (1)(q32). Inducibility by distamycin A, concomitance with fraglile (16)(q22). Hum Genet 73: 232–243
Shabtai F, Orlyn J, Hart J, Halbrecht I, Klar D, Friedman J (1986b) Different inducibility and possible significance of several concomitant “fragile sites” in two brothers. Hum Genet 74:85–89
Tommerup N, Nielsen J, Mikkelsen M (1985) Afolate sensitive heritable fragile site at 19p13. Clin Genet 27:510–514
Yunis JJ, Soreng AL (1984) Constitutive fragile sites and cancer. Science 226:1199–1204
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Fuster, C., Mirò, R., Templado, C. et al. Can sister chromatid intercrossings be considered as prelesions?. Hum Genet 79, 179–180 (1988). https://doi.org/10.1007/BF00280561
- Internal Medicine
- Metabolic Disease
- RPMI Medium
- Normal Individual