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The NADH dehydrogenase subunit 7 gene is interrupted by four group II introns in the wheat mitochondrial genome

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We have characterized a wheat mitochondrial gene, designated nad7, capable of encoding a 394-amino acid subunit of the respiratory chain NADH dehydrogenase complex. It contains four introns possessing group II features and their positions differ from those in both the liverwort mitochondrial nad7 pseudogene and the nuclear gene encoding the homologous 49 kDa subunit of complex I in Neurospora. The derived amino acid sequence of the wheat nad7 gene is strongly conserved relative to its nuclear or organellar counterparts in other organisms. C-to-U type RNA editing, which is observed at 32 positions within the coding region of wheat nad7 transcripts, strengthens protein sequence similarity. RNA editing is also predicted to improve base-pairing within the domain V/VI regions of all four introns.

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  1. Arizmendi JM, Runswick MJ, Skehel JM, Walker JE (1992) NADH:ubiquinone oxidoreductase from bovine heart mitochondria: a fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes. FEBS Lett 301:237–242

  2. Binder S, Marchfelder A, Brennicke A, Wissinger B (1992) RNA editing in trans-splicing intron sequences of nad2 mRNAs in Oenothera mitochondria. J Biol Chem 267:7615–7623

  3. Böhm R, Sauter M, Böck A (1990) Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components. Mol Microbiol 4:231–243

  4. Bonen L (1987) The mitochondrial S13 ribosomal protein gene is silent in wheat embryos and seedlings. Nucleic Acids Res 15:10393–10404

  5. Bonen L (1993) Trans-splicing of pre-mRNA in plants, animals and protists. FASEB J 7:40–46

  6. Bonnard G, Gualberto JM, Lamattina L, Grienenberger JM (1992) RNA editing in plant mitochondria. Crit Rev Plant Sci 10:503–524

  7. Brandt P, Sunkel S, Unseld M, Brennicke A, Knoop V (1992) The nad4L gene is encoded between exon c of nad5 and orf25 in the Arabidopsis mitochondrial genome. Mol Gen Genet 236:33–38

  8. Chapdelaine Y, Bonen L (1991) The wheat mitochondrial gene for subunit I of the NADH dehydrogenase complex: a trans-splicing model for this gene-in-pieces. Cell 65:465–472

  9. Conklin PL, Wilson RK, Hanson MR (1991) Multiple trans-splicing events are required to produce a mature nad1 transcript in a plant mitochondrion. Genes Dev 5:1407–1415

  10. deVrics S, Grivell LA (1988) Purification and characterization of a rotenone-insensitive NADH: Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae. Eur J Biochem 176:377–384

  11. Douce R, Neuburger M (1989) The uniqueness of plant mitochondria. Annu Rev Plant Physiol Plant Mol Biol 40:371–414

  12. Fearnley IM, Runswick MJ, Walker JE (1989) A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA. EMBO J 8:665–672

  13. Gäbler L, Herz U, Liddell A, Leaver CJ, Schröder W, Brennicke A, Grohmann L (1994) The 42.5 kDa subunit of the NADH: ubiquinone oxidoreductase (complex 1) is in higher plants encoded by the mitochondrial nad7 gene. Mol Gen Genet 243:

  14. Gass DA, Makaroff CA, Palmer JD (1992) Variable intron content of the NADH dehydrogenase subunit 4 gene of plant mitochondria. Curr Genet 21:423–430

  15. Gray MW (1992) The endosymbiotic hypothesis revisited. Int Rev Cytol 141:233–357

  16. Gray MW, Covello PS (1993) RNA editing in plant mitochondria and chloroplasts. FASEB J 7:64–71

  17. Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are co-transcribed. Mol Gen Genet 215:118–127

  18. Haouazine N, Pereira de Souza A, Jubier MF, Lancelin D, Delcher E, Lejeune B (1992) The wheat mitochondrial genome contains an ORF showing sequence homology to the gene encoding the subunit 6 of the NADH-ubiquinone oxidoreductase. Plant Mot Biol 20:395–404

  19. Knoop V, Schuster W, Wissinger B, Brennicke A (1991) Trans splicing integrates an exon of 22 nucleotides into the nad5 mRNA in higher plant mitochondria. EMBO J 10: 3483–3493

  20. Koch JL, Boulanger SC, Dib-Hajj SD, Hebbar SK, Perlman PS (1992) Group II introns deleted for multiple substructures retain self-splicing activity. Mol Cell Biol 12:1950–1958

  21. Koslowsky DJ, Bhat GJ, Perrollaz AL, Feagin JE, Stuart K (1990) The MURF3 gene of T.brucei contains multiple domains of extensive editing and is homologous to a subunit of NADH dehy-drogenase. Cell 62:901–911

  22. Lamattina L, Grienenberger JM (1991) RNA editing of the transcript coding for subunit 4 of NADH dehydrogenase in wheat mitochondria: uneven distribution of the editing sites among the four exons. Nucleic Acids Res 19: 3275–3282

  23. Lejeune B, Quetier F (1988) Structure de l'ADN mitochondrial de blé. In: Variabilité génétique cytoplasmique et sterilité male cytoplasmique. INRA Colloques No 45, Paris, pp 201–207

  24. Matsubayashi T, Wakasugi T, Shinozaki K, Yamaguchi-Shinozaki K, Zaita N, Hidaka T, Meng BY, Ohto C, Tanaka M, Kato A, Maruyama T, Sugiura M (1987) Six chloroplast genes (ndhA-F) homologous to human mitochondrial genes encoding components of the respiratory chain NADH dehydrogenase are actively expressed. Mol Gen Genet 210:385–393

  25. Michel F, Umesono K, Ozeki H (1989) Comparative and functional anatomy of group II catalytic introns — a review. Gene 82: 5–30

  26. Nugent JM, Palmer JD (1993) Characterization of the Brassica campestris mitochondrial gene for subunit six of NADH dehydrogenase: nad6 is present in the mitochondrion of a wide range of flowering plants. Curr Genet 23:148–153

  27. Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama, K (1992) Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. J Mol Biol 223:1–7

  28. Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322: 572–574

  29. Pereira de Souza A, Jubier MF, Delcher E, Lancelin D, Lejeune B (1991) A trans-splicing model for the expression of the tripartite nad5 gene in wheat and maize mitochondria. The Plant Cell 3:1363–1378

  30. Perlman PS, Butow RA (1989) Mobile introns and intron-encoded proteins. Science 246:1106–1109

  31. Preis D, van der Pas JC, Nehls U, Rohlen DA, Sackmann U, Jahnke U, Weiss H (1990) The 49 K subunit of NADH:ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein. Curr Genet 18:59–64

  32. Pritchard AE, Venuti SE, Ghalambor MA, Sable CL, Cummings DJ (1989) An unusual region of Paramecium mitochondrial DNA containing chloroplast-like genes. Gene 78:121–134

  33. Rasmussen J, Hanson MR (1989) A NADH dehydrogenase subunit gene is co-transcribed with the abnormal Petunia mitochondrial gene associated with cytoplasmic male sterility. Mol Gen Genet 215:332–336

  34. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

  35. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

  36. Schuster W, Wissinger B, Unseld M, Brennicke A (1990) Transcripts of the NADH dehydrogenase subunit 3 gene are differentially edited in Oenothera mitochondria. EMBO J 9:263–269

  37. Shaw JM, Campbell D, Simpson L (1989) Internal frame shifts within the mitochondrial genes for cytochrome oxidase subunit II and maxicircle unidentified reading frame 3 of Leishmania tarentolae are corrected by RNA editing: evidence for translation of the edited cytochrome oxidase II mRNA. Proc Natl Acad Sci USA 86:6220–6224

  38. Souza AE, Myler PJ, Stuart K (1992) Maxicircle CR1 transcripts of Trypanosoma brucei are edited and developmentally regulated and encode a putative iron-sulfur protein homologous to an NADH dehydrogenase subunit. Mot Cell Biol 12:2100–2107

  39. van der Spek H, van ben Burg J, Croiset A, van den Broek M, Sloof P, Benne R (1988) Transcripts from the frameshifted MURF3 gene from Crithidia fasciculata are edited by U insertion at multiple sites. EMBO J 7:2509–2514

  40. Walker JE (1992) The NADH: ubiquinone oxidoreductase (complex I) of respiratory chains. Quart Rev Biophys 25: 253–324

  41. Weiss H, Friedrich T, Hofhaus G, Preis D (1991) The respiratorychain NADH dehydrogenase (complex I) of mitochondria. Eur J Biochem 197:563–576

  42. Wissinger B, Schuster W, Brennicke A (1991) Trans splicing in Oenothera mitochondria: nad1 mRNAs are edited in exon and trans-splicing group II intron sequences. Cell 65:473–482

  43. Wissinger B, Brennicke A, Schuster W (1992) Regenerating good sense: RNA editing and trans-splicing in plant mitochondria. Trends Genet 8: 322–328

  44. Xu X, Matsuno-Yagi A, Yagi T (1992) Gene cluster of the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans: characterization of four structural gene products. Biochemistry 31:6925–6932

  45. Zhuo D, Bonen L (1993) Characterization of the S7 ribosomal protein gene in wheat mitochondria. Mol Gen Genet 236:395–401

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Bonen, L., Williams, K., Bird, S. et al. The NADH dehydrogenase subunit 7 gene is interrupted by four group II introns in the wheat mitochondrial genome. Molec. Gen. Genet. 244, 81–89 (1994). https://doi.org/10.1007/BF00280190

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Key words

  • Intron
  • Mitochondrial gene
  • NADH dehydrogenase
  • RNA editing
  • Wheat