Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effect of furosemide on angiotensin II-mediated prostaglandin I2 production in hypertensive subjects


The role of angiotensin II (AII) in Prostaglandin I2 (PGI2) production following furosemide has been examined in a placebo-controlled, cross-over study. Furosemide 20 mg was injected intravenously in eight hypertensive subjects already treated with oral captopril 25 mg or a matching placebo. Urinary excretion of 6-keto-PGF (a metabolite of PGI2) and PGE2, PRA and AII was increased following furosemide without captopril pretreatment. The rises in urinary 6-keto-PGF and PGE2, and plasma AII after furosemide were prevented by the captopril pretreatment. Urinary volume, sodium and furosemide were not affected by captopril. The data indicate that the effect of furosemide on PGI2 production, as reflected by the urinary excretion of 6-keto-PGF, was mediated by an action of AII.

This is a preview of subscription content, log in to check access.


  1. 1.

    Scherer B, Weber PC (1979) Time-dependent changes in prostaglandin excretion in response to furosemide in man. Clin Sci 56: 77–81

  2. 2.

    Mackay IG, Muir AL, Watson ML (1984) Contribution of prostaglandins to the systemic and renal vascular response to furosemide in normal man. Br J Clin Pharmacol 17: 513–519

  3. 3.

    Fujimura A, Ebihara A (1988) Role of angiotensin II in renal prostaglandin E2 production after furosemide administration. Hypertension 11: 491–494

  4. 4.

    Levenson DJ, Simmons CE, Brenner BM (1982) Arachidonic acid metabolism, prostaglandins and the kidney. Am J Med 72: 354–374

  5. 5.

    Stahl RAK, Paravicini M, Schollmeyer P (1984) Angiotensin II stimulation of prostaglandin E2 and 6-keto-F formation by isolated human glomeruli. Kidney Int 26: 30–34

  6. 6.

    Patrono C, Pugliese F, Ciabattoni G, Patrignani P (1982) Evidence for a direct stimulatory effect of prostacyclin on renin release in man. J Clin Invest 69: 231–239

  7. 7.

    Ikeda I, Iinuma K, Takai M (1982) Measurement of plasma renin activity by a simple solid phase radioimmunoassay. J Clin Endocrinol Metab 54: 423–428

  8. 8.

    Morimoto T, Aoyama M, Gotoh E, Shionoiri H (1983) A method of radioimmunoassay of plasma angiotensin II using florisil. Folia Endocrinol Jpn 59: 215–229

  9. 9.

    MacDougall ML, Shoeman DW, Azarnoff DL (1975) Separation and quantitative analysis of furosemide and 4-chloro-5-sulfamoylanthranilic acid (CSA) by high pressure liquid chromatography. Res Commun Chem Pathol Pharmacol 10: 285–292

  10. 10.

    Kawano K, Sugita M, Oka M, Tabata N (1987) A simple, rapid and simultaneous extraction of thromboxane B2, 6-keto-prostaglandin F and prostaglandin E2. Jpn J Inflammation 7: 511–515

  11. 11.

    Page IH (1987) The effects of angiotensin on renal circulation. In: Hypertension mechanisms. Grune and Stratton, Orlando, pp 389–393

  12. 12.

    Whittle BJR, Moncada S (1984) Prostacyclin-thromboxane interactions in hemostasis. In: Antonaccio M (ed) Cardiovascular pharmacology, 2nd edn. Raven Press, New York, pp 519–534

  13. 13.

    Brogden RN, Todd PA, Sorkin EM (1988) Captopril; an update of its pharmacodynamic and pharmacokinetic properties, and therapeutic use in hypertension and congestive heart failure. Drugs 36: 540–600

  14. 14.

    Van Hecken AM, Verbesselt R, Buntinx A, Cirillo VJ, De Schepper PJ (1987) Absence of a pharmacokinetic interaction between enalapril and furosemide. Br J Clin Pharmacol 23: 84–87

  15. 15.

    De Lepeleire I, Van Hecken A, Verbesselt R, Kaiser G, Barner A, Holmes I, De Schepper PJ (1988) Interaction between furosemide and the converting enzyme inhibitor benazepril in healthy volunteers. Eur J Clin Pharmacol 34: 465–468

  16. 16.

    Rakhit A, Kochak GM, Tipnis V, Hurley ME (1987) Inhibition of renal clearance of furosemide by pentopril, an angiotensin-converting enzyme inhibitor. Clin Pharmacol Ther 41: 580–586

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fujimura, A., Ebihara, A. Effect of furosemide on angiotensin II-mediated prostaglandin I2 production in hypertensive subjects. Eur J Clin Pharmacol 39, 113–115 (1990).

Download citation

Key words

  • Furosemide
  • 6-keto-PGF
  • hypertension
  • angiotensin II
  • captopril
  • urinary PGs
  • PGI2