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Stability of lectin binding properties expressed by human bladder carcinoma cell lines passaged in vitro or in nude mice

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Binding of a panel of lectins by sublines of two human bladder carcinoma cell lines, UCRU-BL-17 CL and UCRU-BL-13 CL, assessed flow cytometrically following passage of the cells in vitro and in nude mice, was compared with that of human leukaemic cell lines, K562 and HL60, and found to be different. Marked glycosylation of sublines of both bladder cancer cell lines was found compared with normal human bladder transitional epithelium (assessed cytochemically). Neuraminidase pretreatment increased the binding of some lectins indicating that some galactose and N-acetylgalactosamine residues were sialylated. Lectin binding by UCRU-BL-17 CL sublines was remarkably constant on prolonged passage in vitro even though the lines underwent changes in physical characteristics and ploidy when grown in nude mice. This suggests that glycosylation of the tumour cell surface may represent an intrinsic feature of this bladder tumour.

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  1. 1.

    Alroy J, Szoka FC, Heaney JA, Ucci AA (1982) Lectins as a probe for carbohydrates in non-neoplastic urothelium of human urinary bladder. J Urol 128:189–193

  2. 2.

    Chen TR (1977) In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res 104:255–262

  3. 3.

    Coombe DR, Parish CR, Ramshaw IA, Snowden JM (1987) Analysis of the inhibition of tumor metastasis by sulphated polysaccharides. Int J Cancer 39:82–88

  4. 4.

    Debray H, Qin Z, Delannoy P, Montreuil J, Dus D, Radzikowski C, Christensen B, Kieler J (1986) Altered glycosylation of membrane glycoproteins in human uroepithelial cell lines. Int J Cancer 37:607–611

  5. 5.

    Dus D, Radzikowski C, Debray H, Montreuil J, Christensen B, Kieler J (1985) Lectin binding to nonmalignant and malignant human uroepithelial cells in vitro. In: Lectins, vol IV. de Gruyter, Berlin New York, pp 65–74

  6. 6.

    Leathem AJC, Atkins NJ (1983) In: Bullock GR, Petrusz P (eds) Techniques in immuno-cytochemistry, vol 2. Academic Press, New York, pp 40–70

  7. 7.

    Lehman TP, Cooper HS, Mulholland SG (1984) Peanut lectin binding sites in transitional cell carcinoma of the urinary bladder. Cancer 53:272–277

  8. 8.

    Limas C, Lange PH (1985) Lewis antigens in normal and neoplastic urothelium. Am J Pathol 121:176–183

  9. 9.

    Mullins DE, Rohrlich ST (1983) The role of proteinases in cellular invasiveness. Biochim Biophys Acta 695:177–214

  10. 10.

    Nicolson GL (1982) Cancer metastatis: organ colonization and the cell-surface properties of malignant cells. Biochim Biophys Acta 695:113–176

  11. 11.

    Russell PJ, Jelbart ME, Wills E, Singh S, Wotherspoon J, Raghavan D (1988) Establishment and characterization of a new human bladder cancer cell line showing features of squamous and glandular differentiation. Int J Cancer 42:74–82

  12. 12.

    Russell PJ, Raghavan D, Gregory P, Philips J, Wills EJ, Jelbart ME, Wass J, Zbroja RA, Vincent PC (1986) Bladder cancer xenografts: a model of tumor cell heterogeneity. Cancer Res 46:2035–2040

  13. 13.

    Russell PJ, Wills EJ, Philips J, Wass J, Jelbart M, Gregory P, Raghavan D (1988) Features of squamous and adeno-carcinoma in the same cell in a xenografted human transitional cell carcinoma: evidence of a common histogenesis? Urol Res 16:79–85

  14. 14.

    Smets LA, van Beek WP (1984) Carbohydrate of the tumour cell surface. Biochim Biophys Acta 738:237–249

  15. 15.

    Steele JG, Rowlett C, Sandall JK, Franks LM (1983) Identification of exposed surface glycoproteins of four human bladder cancer cell lines. Biochim Biophys Acta 732:219–228

  16. 16.

    Walker KZ, Hayden GE, Goodnow CC, Adams E, Basten A, Raison RL (1985) Coexpression of an epitope on human free k light chains and on a cytoplasmic component in activated T cells. J Immunol 134:1059–1064

  17. 17.

    Yuan M, Itzkowitz SH, Boland CR, Kim YD, Tomita JT, Palekar A, BenningtonJL, Trump BF, Kim YS (1986) Comparison of T antigen expression in normal, premalignant and malignant colonic tissue using lectin and antibody immunohistochemistry. Cancer Res 46:4841–4847

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Russell, P.J., Wotherspoon, J., Jelbart, M. et al. Stability of lectin binding properties expressed by human bladder carcinoma cell lines passaged in vitro or in nude mice. Urol. Res. 16, 407–414 (1988). https://doi.org/10.1007/BF00280020

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Key words

  • Lectin binding
  • Human bladder TCC cell lines
  • Flow cytometry