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An internal AUU codon initiates a smaller peptide encoded by bacteriophage T4 baseplate gene 26

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Bacteriophage T4 baseplate gene 26 codes for two in-frame overlapping peptides with identical C-terminal regions. By site-directed mutagenesis we have now determined that an internal AUU, codon 114 of gene 26, is used as the initiation codon for the synthesis of a smaller peptide (gp26*). Thus gene 26* gives rise to a peptide of 95 amino acid residues with an Mr of 10873, while the complete gene 26 encodes a peptide of 208 amino acid residues of Mr 23 880. Expression of gene 26* is shown to depend on the RNA secondary structure in the translational initiation region of this gene.

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Correspondence to Rimas Nivinskas.

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Communicated by H. Hennecke

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Nivinskas, R., Vaiškunaite, R. & Raudonikiene, A. An internal AUU codon initiates a smaller peptide encoded by bacteriophage T4 baseplate gene 26. Molec. Gen. Genet. 232, 257–261 (1992). https://doi.org/10.1007/BF00280004

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Key words

  • Overlapping genes
  • Internal initiation codon
  • RNA secondary structure
  • T4 late promoters