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Cloning and mapping of the genetic determinants for microcin C51 production and immunity

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Microcin C51 is a small peptide antibiotic produced by Escherichia coli cells harbouring the 38 kb low copy number plasmid pC51, which codes for microcin production and immunity. The genetic determinants for microcin synthesis and immunity were cloned into the vectors pBR325, pUC19 and pACYC184. Physical and phenotypic analysis of deletion derivatives and mutant plasmids bearing insertions of transposon Tn5 showed that a DNA fragment of about 5 kb is required for microcin C51 synthesis and expression of complete immunity to microcin. Partial immunity can be provided by a 2 kb DNA fragment. Mutant plasmids were tested for their ability to complement Mic mutations. Results of these experiments indicate that at least three plasmid genes are required for microcin production. The host OmpR function is also necessary for microcin C51 synthesis.

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  1. Asensio C, Perez-Dias JC, Martinez MC, Baquero F (1976) A new family of low molecular weight antibiotics from enterobacteria. Biochem Biophys Res Commun 69:7–14

  2. Baquero F, Moreno F (1984) The microcins. FEMS Microbiol Lett 23:117–124

  3. Davagnino J, Herrero M, Furlong D, Moreno F, Kolter R (1986) The DNA replication inhibitor microcin B17 is a forty-three-amino-acid protein containing sixty percent glycine. Proteins 1:230–238

  4. Garcia-Bustos JF, Pezzi N, Asensio S (1984) Microcin 7: purification and properties. Biochem Biophys Res Commun (1984) 119:779–785

  5. Garcia-Bustos JF, Pezzi N, Mendez E (1985) Structure and mode of action of microcin 7, an antibacterial peptide produced by Escherichia coli. Antimicrob Agents Chemother 27:791–797

  6. Genilloud O, Moreno F, Kolter R (1989) DNA sequence, products, and transcriptional pattern of the genes involved in production of the DNA replication inhibitor microcin B17. J Bacteriol 171:1126–1135

  7. Hirsch PR (1979) Plasmid-determined bacteriocin production by Rhizobium leguminosarum. J Gen Microbiol 113:219–228

  8. Khmel IA, Kopylov VM, Vorobjeva IP, Polyanin VP (1981) The influence of colicinogenic plasmids Col1b-P9, Col1a-CA53 and ColV-K30 on the repair, mutagenesis and induction of colicin E1 synthesis. Mol Gen Genet 181:101–106

  9. Khmel IA, Lipasova VA, Kurepina NE, Rekesh AN, Kolot MN (1987) The nature of typhimuricin, the antibiotic substance produced by Salmonella typhimurium LT2. Microbios Lett 36:33–38

  10. Lavina M, Gaggero C, Moreno F (1990) Microcin H47, a chromosome encoded microcin antibiotic of Escherichia coli. J Bacteriol 172:6585–6588

  11. Lavina M, Pugsley AP, Moreno F (1986) Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12. J Gen Microbiol 132:1685–1693

  12. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold spring Harbor, New York

  13. Martinez JL, Perez-Diaz JC (1990) Cloning of the determinants for microcin D93 production and analysis of three different D-type microcin plasmids. Plasmid 23:216–225

  14. Novoa MA, Diaz-Guerra L, San Millan JL, Moreno F (1986) Cloning and mapping of the genetic determinants for microcin C7 production and immunity. J Bacteriol 168:1384–1391

  15. Pugsley AP (1984) The ins and outs of colicins. Microbiol. Sci. 1:168–175

  16. Pugsley AP (1985) Escherichia coli K12 strains for use in the identification and characterization of colicins. J Gen Microbiol 131:369–379

  17. Shanabruch WG, Walker GC (1980) Localization of the plasmid (pKM101) gene(s) involved in recA + lexA +-dependent mutagenesis. Mol Gen Genet 179:289–297

  18. Yankovsky NK, Fonstein MJu, Lashina SJu, Ermakova LM, Rebentisch BA, Debabov VG. Construction and usage of large capacity phasmid vector molecules. (1987) Molec Biol (Russian) 21:831–836

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Correspondence to Inessa A. Khmel.

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Communicated by W. Goebel

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Kurepina, N.E., Basyuk, E.I., Metlitskaya, A.Z. et al. Cloning and mapping of the genetic determinants for microcin C51 production and immunity. Molec. Gen. Genet. 241, 700–706 (1993). https://doi.org/10.1007/BF00279914

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Key words

  • Microcin C51
  • Peptide antibiotic
  • Plasmid genes
  • Escherichia coli