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Distribution of constitutive heterochromatin in Hela and HEp-2 cell lines

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Summary

Despite the fact that HeLa and HEp-2 cell lines have been established respectively from a female and a male patient, the frequent loss of the Y chromosome makes it difficult to discriminate the two cell types. The technique of centromeric localization of heterochromatin, however, facilitates more definite identification of the two cell types. Giemsa positive centromeric constitutive heterochromatin blocks were induced within the intact metaphase chromosome preparations of Hela and HEp-2 cells. The Hela cell population had a single heterochromatin marker chromosome, whereas the HEp-2 cell line was found to possess two types of heterochromatin markers. The distinctive patterns of distribution of constitutive heterochromatin of these three chromosomes served as the guides in tracing the presumptive cytological pathways for their orgin.

Zusammenfassung

Trotz der Tatsache, daß Hela- und HEp-2-Zellinien jeweils von einem weiblichen und einem männlichen Patienten stammen, erschwert der häufige Verlust des Y-Chromosoms eine Unterscheidung der beiden Zelltypen. Der Nachweis des zentromernahen Heterochromatins jedoch ermöglicht eine zuverlässigere Erkennung dieser beiden Zelltypen. Die Hela-Zellpopulation hat ein einzelnes, durch das Heterochromation gekennzeichnetes Marker-Chromosom, während die HEp-2-Zellinie deren 2 besitzt. Das distinkte Verteilungsmuster des konstitutiven Heterochromatons dieser 3 Chromosomen dient dazu, die vermutliche cytologische Herkunft zu interpretieren.

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Supported in part by the Robert A. Welch Foundation, The National Foundation —March of Dimes and USPHS Grant No. FR-05425.

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Sinha, A.K., Pathak, S. Distribution of constitutive heterochromatin in Hela and HEp-2 cell lines. Hum Genet 18, 47–54 (1973). https://doi.org/10.1007/BF00279030

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Keywords

  • Cell Population
  • Hela Cell
  • Male Patient
  • Distinctive Pattern
  • Metaphase Chromosome