Advertisement

Histochemie

, Volume 30, Issue 2, pp 247–254 | Cite as

Sur les réactions histochimiques des radicaux acides forts et notemment phosphate

  • Guido Palladini
Article
  • 10 Downloads

Résumé

L'auteur a étudié la spécificité des réactions considérées spécifiques des radicaux acides sur des modèles obtenus par sulfatation, phosphatation et carboxylation des diverses structures riches en groupes vic-glycols.

L'auteur a ainsi démontré que toutes les réactions routinaires pour la visualisation des groupes sulfat sont aspécifiques, étant donné leur positivité aussi envers les radicaux phosphate.

L'auteur démontre, au contraire, la spécificité de deux réactions, respectivement pour les radicaux sulfat et phosphate, déjà connues mais oubliées, et en propose des modifications.

On the histochemical reactions of strong acid radicals with special reference to phosphate groups

Summary

The specificity of the reactions which are presumed to be specific for acid radicals have been tested in model experiments by means of sulphatation, phosphorylation and carboxylation of structures being rich in vic-glycol groups. The non-specifity of all routine reactions for sulphate groups is proved because they are positive also for phosphate groups. Two other reactions were modified resulting in specifity for sulphate and phosphate groups, respectively.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliographie

  1. Adams, C. W. M.: Neurohistochemistry. Amsterdam-London-New York: Elsevier 1965.Google Scholar
  2. Battaglia, C.: Studio istofotometrico della metacromasia. a vari pH sulla medesima sezione istologica. Riv. Istoch. 10, 621–644 (1964).Google Scholar
  3. Collins, G. H., Cowden, R. R., Nevis, A. H.: Myoolonus epilepsy with Lafora bodies. Arch. Path. 86, 239–254 (1968).Google Scholar
  4. Curran, R. C.: The histochemistry of mucopolysaccharides. Int. Rev. Cytol. 17, 149–212 (1964).Google Scholar
  5. Dubois-Dalcq, M.: Etude histochimique des corps de Lafora. Acta neuropath. (Berl.) 12, 205–217 (1969).Google Scholar
  6. Fischer, E. R., Lillie, R. D.: The effect of methylation on basophilia. J. histochem. Cytochem. 2, 81–87 (1954).Google Scholar
  7. Gabe, M.: Techniques histologiques. Paris: Masson 1968.Google Scholar
  8. Ganter, P., Jolles, G.: Histochimie normale et pathologique. Paris: Gauthier-Villars 1969.Google Scholar
  9. Geyer, G.: Der Sulfonsäurenachweis. Eine neue Methode für den histochemischen Nachweis von Sulfonsäuren. Acta histochem. (Jena) 14, 26–41 (1962).Google Scholar
  10. Glegg, R. E., Clermont, J., Leblond, C. P.: The use of lead tetraacetate, benzidine, o-di-anisidine and a “film test” in the investigating the periodic Schiff technique. Stain Technol. 27, 277–305 (1952).Google Scholar
  11. Handbook of chemistry and physics R. C. Weast, Selbi, S. M., eds.). Cleveland: Chemical Rubber 1966–1967).Google Scholar
  12. Haust, D. M., Landing, B. H.: Analysis of reaction of phosphoryl chloride with tissue components. J. Histochem. Cytochem. 9, 548–552 (1961).Google Scholar
  13. Heikop ten Ham, M. W.: Lafora disease: review of histochemical aspects. Arch. Neurobiol. (Madr.) 28, 647–666 (1965).Google Scholar
  14. Korhonen, L. K.: Specific methylation of carboxyl groups by thyonil chloride in methanol. Acta histochem. (Jena) 26, 80–86 (1967).Google Scholar
  15. Landing, B. H., Hall, H. E.: Phosphorylation as a histochemical procedure. J. Histochem. Cytochem. 4, 41–46 (1956).Google Scholar
  16. Landsmeer, J. M. F.: Some colloid chemical aspects of methachromasia; influence of pH and salts on metachromatic phenomena evoked by toluidine blue in animal tissue. Acta pharmacol. neerl. 2, 112–128 (1951).Google Scholar
  17. Lillie, R. D.: Histopathologic technic and practical histochemistry. New York: McGraw-Hill 1965.Google Scholar
  18. Lison, L.: Histochemie et cytochemie animales. Paris: Gauthiers-Villars 1960.Google Scholar
  19. Luft, J. H.: Ruthenium red and violet. I∘: chemistry; purification methods of use for electron microscopy and mechanism of action. Anat. Rec. 171, 347–369 (1971a).Google Scholar
  20. Luft, J. H.: II∘: fine structural localisation in animal tissue. Anat. Rec. 171, 369–417 (1971b).Google Scholar
  21. Mangin: L.: Sur l'emploi du rouge de ruthénium en anatomie vegetale. C. R. Acad. Sci. (Paris) 66, 363–365 (1893).Google Scholar
  22. Marchetti, G.: Bario. In: Nuova Enc. Chimica (I. Guareschi, ed.), T. 4, p. 706–733. Torino: Utet 1924.Google Scholar
  23. Martin, J. J., Barcy, Th. de, Palladini, G.: La maladie de Pompe: Etude morphologique des lesions du système nerveux et des muscles striés (sous presse).Google Scholar
  24. Mayer, P.: Über Schleinmfärbung. Mitt. zool. Stat. Neapel 12, 303–330 (1896).Google Scholar
  25. Mowry, R. W.: Improved procedure for the staining of acidic polysaccharides by Muller's colloidal (hydrous) ferric oxyde and its combination with the Feulgen and the periodic Schiff reactions. Lab. Invest. 7, 566–576 (1958a).Google Scholar
  26. Mowry, R. W.: Observations on the use of sulfuric acid in ether for the sulfatation of hydroxyl groups in tissue sections. J. Histochem. Cytochem. 6, 82–83 (1958b).Google Scholar
  27. Mowry, R. W.: The special value of methods that color both acidic and vicinal hydroxyl groups in the histochemical study of mucins. With revised directions for the colloidal iron stain, the use of Alcian blue 8GX and their combination with the periodic Schiff reaction. Ann. N.Y. Acad. Sci. 106, 402–423 (1963).Google Scholar
  28. Palladini, G., Alfei, L.: Istochimica dei mucopolisaccaridi solforati: la reazione di Bracco-Curti. Riv. Istoch. 11, 51–60 (1965).Google Scholar
  29. Pearse, A. G. E.: Histochemistry, theoretical and applied. London: J. A. Churchill 1968.Google Scholar
  30. Rizzotti, M.: Sulla cromotropia di un polisaccaride fosforato presente nel secreto della ghiandola dell'albume di Helix terrestris. Riv. Istoch. 8, 287–293 (1962).Google Scholar
  31. Sakai, M., Austin, J., Witmer, F., Trueb, L.: Studies in myoclonus epilepsy (Lafora body form). II∘ Polyglucosans in the systemic deposits of myoclonus epilepsy and in corpora amylacea. Neurology (Minneap.) 20, 160–176 (1970).Google Scholar
  32. Spicer, S. S.: A correlative study of the histochemioal properties of rodent acid mucopolysaocharides. J. Histochem. Cytochem. 8, 18–35 (1960).Google Scholar
  33. Stempien, M. F.: Localisation of acid mucopolysaccharides with basic fuchsin. J. Histochem. Cytochem. 10, 766 (1962).Google Scholar
  34. Stempien, M. F.: The use of a barium chloride-rhodizonate technique for the histochemical localisation of some sulphated acid mucopolysaccharides. J. Histochem. Cytochem. 11, 478–481 (1963).Google Scholar
  35. Vilter, V.: Contribut à l'étude du mécanisme de la “méthylation saponification” dans l'histo-chimie des mucines acides. Ann. Histochem. 13, 205–220 (1968).Google Scholar
  36. Warren, L., Spicer, S. S.: Biochemical and histochemical identification of sialic acid containing mucins of rode vagina and salivary gland. J. Histochem. Cytochem. 9, 400–408 (1961).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Guido Palladini
    • 1
  1. 1.Institut d'Anatomie Comparée “G. B. Grassi”Université de RomeItaly

Personalised recommendations