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Induction of DNA breakdown and death in Escherichia coli by phleomycin

Its association with dark-repair processes

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Summary

Phleomycin, at concentrations above 1 μg/ml, induced breakdown of DNA and death in E. coli. Exponentially growing cultures were about 10 times more sensitive to phleomycin than were stationary cultures, and the effect was somewhat dependent on the medium.

Excisionless (HCR) mutants of E. coli were insensitive to doses of phleomycin which killed over 99% of wild-type organisms within an hour, while EXR mutants were considerably more sensitive.

Mutants of E. coli selected for phleomycin resistance were unable to reactivate U.V. irradiated Tl phage (HCR).

It is concluded that the DNA breakdown, inhibition of DNA replication and cell death are a consequence of initial attack by an excision-endonuclease stimulated by the phleomycin.

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References

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Communicated by R. Pritchard

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Grigg, G.W. Induction of DNA breakdown and death in Escherichia coli by phleomycin. Molec. Gen. Genet. 104, 1–11 (1969). https://doi.org/10.1007/BF00277357

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Keywords

  • Cell Death
  • Stationary Culture
  • Initial Attack
  • Phleomycin Resistance