gdhA1 is a spontaneous mutant of Escherichia coli that causes complete loss of activity of the NADP-specific glutamate dehydrogenase (GDH) encoded by the gdhA gene. The gdhA1 mutational site has been identified by recombinational mapping, polymerase chain reaction (PCR) amplification and DNA sequencing, as an A to G transition at nucleotide 274 of the gdhA coding sequence, resulting in an amino acid change of lysine 92 to glutamic acid. The mutant enzyme forms hybrid hexamers with a wild-type GDH, providing a useful system for analysis of conformational integrity of mutational variants.
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Communicated by J. Lengeler
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Jones, K.M., McPherson, M.J., Baron, A.J. et al. The gdhA1 point mutation in Escherichia coli K12 CLR207 alters a key lysine residue of glutamate dehydrogenase. Molec. Gen. Genet. 240, 286–289 (1993). https://doi.org/10.1007/BF00277068
- gdhA1 point mutation
- Deletion analysis
- Recombination frequency
- DNA sequencing