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Cloning and characterization of an ftsZ homologue from a bacterial symbiont of Drosophila melanogaster

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Abstract

A 1194 by open reading frame that codes for a 398 amino acid peptide was cloned from a λgt11 library of Drosophila melanogaster genomic DNA. The predicted peptide sequence is very similar to three previously characterized protein sequences that are encoded by the ftsZ genes in Escherichia coli, Bacillus subtilis and Rhizobium meliloti. The FtsZ protein has a major role in the initiation of cell division in prokaryotic cells. Using a tetracycline treatment that eradicates bacterial parasites from insects, the ftsZ homologue has been found to be derived from a bacterium that lives within the strain. However, polymerase chain reaction (PCR) amplification of the gene from treated embryos suggests that it is not derived from a gut bacterium. Nevertheless, by amplifying and characterizing part of the 16S rRNA from this bacterium we have been able to demonstrate that it is a member of the genus Wolbachia, a parasitic organism that infects, and disturbs the sexual cycle of various strains of Drosophila simulans. We suggest that this ftsZ homologue is implicated in the cell division of Wolbachia, an organism that fails to grow outside the host organism. Sequence and alignment analysis of this ftsZ homologue show the presence of a potential GTP-binding motif indicating that it may function as a GTPase. The consequences of this function particularly with respect to its role in cell division are discussed.

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Author information

Correspondence to Peter R. Holden.

Additional information

Communicated by D. Finnegan

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Holden, P.R., Brookfield, J.F. & Jones, P. Cloning and characterization of an ftsZ homologue from a bacterial symbiont of Drosophila melanogaster . Molec. Gen. Genet. 240, 213–220 (1993). https://doi.org/10.1007/BF00277059

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Key words

  • ftsZ
  • Wolbachia
  • Drosophila melanogaster
  • Tetracycline