Advertisement

Molecular and General Genetics MGG

, Volume 240, Issue 2, pp 206–212 | Cite as

Multiple pyruvate decarboxylase genes in maize are induced by hypoxia

  • Virginia M. Peschke
  • Martin M. Sachs
Article

Abstract

Two cDNA clones corresponding to anaerobically induced maize mRNAs were found to have homology to a previously identified maize pyruvate decarboxylase gene. DNA sequencing and RFLP mapping indicate that these cDNAs represent two additional maize pdc genes. Each of the clones is approximately 85% identical in predicted amino acid sequence to the other two. All three clones are induced by hypoxic stress, but with different levels and kinetics of induction.

Key words

Anaerobiosis Hypoxia Maize Pyruvate decarboxylase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burr B, Burr FA (1991) Recombinant inbreds for molecular mapping in maize: theoretical and practical considerations. Trends Genet 7:55–60Google Scholar
  2. Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526Google Scholar
  3. Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995Google Scholar
  4. Davies DD, Grego S, Kenworthy P (1974) The control of the production of lactate and ethanol by higher plants. Planta 118:297–310Google Scholar
  5. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: Version 2. Plant Mol Biol Rep 1:19–21Google Scholar
  6. Dennis ES, Gerlach WL, Pryor AJ, Bennetzen JL, Inglis A, Llewellyn D, Sachs MM, Ferl RJ, Peacock WJ (1984) Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res 12:3983–4000Google Scholar
  7. Feinberg AP, Vogelstein B (1984) Addendum: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267Google Scholar
  8. Freeling M (1973) Simultaneous induction by anaerobiosis or 2,4-D of multiple enzymes specified by two unlinked genes: differential Adh1-Adh2 expression in maize. Mol Gen Genet 127:215–227Google Scholar
  9. Gardiner JM, Coe EH, Melia-Hancock S, Hoisington DA, Chao S. Development of a core RFLP map in maize using an immortalized F2 population. Genetics, in pressGoogle Scholar
  10. Gounaris AD, Turkenkopf I, Civerchia LL, Greenlie J (1975) Pyruvate decarboxylase. III: Specificity restrictions for thiamine pyrophosphate in the protein association step, subunit structure. Biochim Biophys Acta 405:492–499Google Scholar
  11. Kelley PM (1989) Maize pyruvate decarboxylase mRNA is induced anaerobically. Plant Mol Biol 13:213–222Google Scholar
  12. Kelley PM, Godfrey K, Lal SK, Alleman M (1991) Characterization of the maize pyruvate decarboxylase gene. Plant Mol Biol 17:1259–1261Google Scholar
  13. Lal SK, Johnson S, Conway T, Kelley PM (1991) Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli. Plant Mol Biol 16:787–795Google Scholar
  14. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181Google Scholar
  15. Laszlo A (1981) Characterization of an inducible enzyme: maize pyruvate decarboxylase. PhD thesis, University of California, Berkeley, California, USAGoogle Scholar
  16. Laszlo A, St Lawrence P (1983) Parallel induction of PDC and ADH in anoxic maize roots. Mol Gen Genet 192:110–117Google Scholar
  17. Leblova S, Malik M, Fojta M (1989) Isolation and characterization of maize pyruvate decarboxylase. Biologia (Bratislava) 44:329–337Google Scholar
  18. Lee TC, Langston-Unkefer PJ (1985) Pyruvate decarboxylase from Zea mays L. I. Purification and partial characterization from mature kernels and anaerobically treated roots. Plant Physiol 79:242–247Google Scholar
  19. Lemke-Keyes CA, Sachs MM (1989a) Anaerobic tolerant null: A mutant that allows Adh1 nulls to survive anaerobic treatment. J Hered 80:316–319Google Scholar
  20. Lemke-Keyes CA, Sachs MM (1989b) Genetic variation for seedling tolerance to anaerobic stress in maize germplasm. Maydica 34:329–337Google Scholar
  21. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  22. Martinez P, Martin W, Cerff R (1989) Structure, evolution and anaerobic regulation of a nuclear gene encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from maize. J Mol Biol 208:551–565Google Scholar
  23. Morrell S, Greenway H, Davies DD (1990) Regulation of pyruvate decarboxylase in vitro and in vivo. J Exp Bot 41:131–139Google Scholar
  24. Oba K, Uritani I (1975) Purification and characterization of pyruvate decarboxylase from sweet potato roots. J Biochem 77:1205–1213Google Scholar
  25. Olive MR, Peacock WJ, Dennis ES (1991) The anaerobic responsive element contains two GC-rich sequences essential for binding a nuclear protein and hypoxic activation of the maize Adh1 promoter. Nucleic Acids Res 19:7053–7060Google Scholar
  26. Rivoal J, Ricard B, Pradet A (1990) Purification and partial characterization of pyruvate decarboxylase from Oryza sativa L. Eur J Biochem 194:791–797Google Scholar
  27. Roberts JKM, Callis J, Wemmer D, Walbot V, Jardetzky O (1984) Mechanism of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia. Proc Natl Acad Sci USA 81:3379–3383Google Scholar
  28. Russell DA, Sachs MM (1989) Differential expression and sequence analysis of the maize glyceraldehyde-3-phosphate dehydrogenase gene family. Plant Cell 1:793–803Google Scholar
  29. Russell DA, Sachs MM (1991) The maize glyceraldehyde-3-phosphate dehydrogenase gene family: organ-specific expression and genetic analysis. Mol Gen Genet 229:219–228Google Scholar
  30. Sachs MM (1991) Molecular response to anoxic stress in maize. In: Jackson MB, Davies DD, Lambers H (eds) Plant life under oxygen deprivation. SPB Academic Publishing, The Netherlands, pp 129–139Google Scholar
  31. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley. Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018Google Scholar
  32. Schwartz D (1969) An example of gene fixation resulting from selective advantage in suboptimal conditions. Am Nat 103:479–481Google Scholar
  33. Springer B, Werr W, Starlinger P, Bennett DC, Zokolica M, Freeling M (1986) The Shrunken gene on chromosome 9 of Zea mays L. is expressed in various plant tissues and encodes an anaerobic protein. Mol Gen Genet 205:461–468Google Scholar
  34. VanToai TT, Fausey NR, McDonald MB Jr (1985) Alcohol dehydrogenase and pyruvate decarboxylase activities in flood-tolerant and susceptible corn seeds during flooding. Agron J 77:753–757Google Scholar
  35. Walker JC, Howard EA, Dennis ES, Peacock WJ (1987) DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci USA 84:6624–6628Google Scholar
  36. Waters I, Morrell S, Greenway H, Colmer TD (1991) Effects of anoxia on wheat seedlings. II. Influence of O2 supply prior to anoxia on tolerance to anoxia, alcoholic fermentation, and sugar levels. J Exp Bot 42:1437–1447Google Scholar
  37. Wignarajah K, Greenway H (1976) Effect of anaerobiosis on activities of alcohol dehydrogenase and pyruvate decarboxylase in roots of Zea mays. New Phytol 77:575–584Google Scholar
  38. Zehender H, Trescher D, Ullrich J (1987) Improved purification of pyruvate decarboxylase from wheat germ: its partial characterisation and comparison with the yeast enzyme. Eur J Biochem 167:149–154Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Virginia M. Peschke
    • 1
  • Martin M. Sachs
    • 2
  1. 1.Department of BiologyWashington UniversitySt. LouisUSA
  2. 2.USDA/ARS, Plant Physiology and Genetics Research Unit and Department of AgronomyUniversity of IllinoisUrbanaUSA

Personalised recommendations