Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Trisomics from triploid-diploid crosses in self-incompatible Lycopersicum peruvianum

I. Essential features of aneuploids and of self-compatible trisomics

  • 43 Accesses

  • 13 Citations


An attempt was carried out to produce trisomics of the wild tomato L. peruvianum, to define their essential features, and to detect relationships between trisomy and the expression of self-compatibility.

Triploid-diploid crosses in L. peruvianum yielded nearly 40% aneuploids. Of these, 18% were single trisomics, and the rest had 2, 3 and 4 extra chromosomes. Almost all the trisomics occurred in crosses where the triploid was used as female parent. Vigour and fertility of trisomics were not much different from those of disomics, and morphologically they were very similar.

The extra chromosome was identified in three self-compatible trisomic plants through somatic and pachytene chromosome morphology. One of these plants was trisomic for chromosome 1, while the other two were trisomic for chromosome 3. In these trisomics a positive correlation was found between chromosome length and trivalent formation, but no relationship between chromosome length and frequency of laggards was observed.

A series of test-crosses revealed that the capacity of the trisomics to produce seed upon selfing always resulted from alterations of the incompatibility phenotype of the style and not from competitive interaction in the pollen. Progeny analyses showed that the self-compatibility features of the trisomics were not transmitted from one generation to the next. The implications of these findings are discussed.

This is a preview of subscription content, log in to check access.


  1. Brewbaker, J.L.; Natarajan, A.T.: Centric fragments and pollenpart mutation of incompatibility alleles in Petunia. Genetics 45, 699–704 (1960)

  2. Burnham, C.R.: Discussions in cytogenetics. 375 pp. Minneapolis, Minnesota: Burgess 1962

  3. Chen, C.C.; Grant, W.F.: Morphological and cytological identification of the primary trisomics of Lotus pedunculatus (Leguminosae). Can. J. Genet. Cytol. 10, 161–179 (s1968a)

  4. Chen, C.C.; Grant, W.F.: Trisomic transmission in Lotus pedunculatus. Can. J. Genet. Cytol. 10, 648–654 (1968b)

  5. Dhillon, T.S.; Garber, E.D.: The genus Collinsia. X. Aneuploidy in C. heterophylla. Bot. Gaz. 121, 125–133 (1960)

  6. Ecochard, R.; Merx, G.: A primary monosomic for chromosome 5 in the tomato. Caryologia 25, 531–536 (1972)

  7. Einset, J.: Chromosome length in relation to transmission frequency of maize trisomes. Genetics 28, 349–364 (1943)

  8. Gill, B.S.; Virmani, S.S.; Minocha, J.L.: Primary simple trisomics in pearl millet. Can. J. Genet. Cytol. 12, 474–483 (1970)

  9. Goodspeed, T.H.; Avery, P.: Trisomic and other types in Nicotiana sylvestris. J. Genet. 38, 381–458 (1939)

  10. Hermsen, J.H.Th.; Wagenvoort, M.; Ramanna, M.S.: Aneuploids from natural and colchicine induced autotetraploids of Solamun. Can. J. Genet. Cytol. 12, 601–613 (1970)

  11. Ising, G.: Cytogenetic studies in Cyrtanthus. II. Aneuploidy and internal chromosome balance. Hereditas 61, 45–115 (1969)

  12. Khush, G.S.: Cytogenetics of aneuploids. 301 pp. New York and London: Academic Press 1973

  13. Lewis, D.: Competition and dominance of incompatibility alleles in diploid pollen. Heredity 1, 85–108 (1947)

  14. Lewis, D.: Structure of the incompatibility gene. II. Induced mutation rate. Heredity 3, 339–355 (1949)

  15. Mooring, J.S.: A cytogenetic study of Clarkia unguiculata II. Supernumerary chromosomes. Amer. J. Botany 47, 847–854 (1960)

  16. Nettancourt, D, de; Ecochard, D.; Perquin, M.D.G.; van der Drift, T.; Westerhof, M.: The generation of new S alleles at the incompatibility locus of Lycopersicum peruvianum Mill. Theor. Appl. Genet. 41, 120–129 (1971)

  17. Nettancourt, D. de; Devreux, M.; Bozzini, A.; Cresti, M.; Pacini, E.; Sarfatti, G.: Ultrastructural aspects of the self-incompatibility mechanism in Lycopersicum peruvianum Mill. J. Cell Sci. 12, 403–419 (1973)

  18. Pandey, K.K.: Mutations of the self-incompatibility gene (s) and pseudo-compatibility in angiosperms. Lloydia 22, 222–234 (1959)

  19. Pandey, K.K.: Elements of the S-gene complex. II. Mutation and complementation at the SI locus in Nicotiana alata. Heredity 22, 255–284 (1967)

  20. Rick, C.M.; Barton, D.W.: Cytological and genetical identification of the primary trisomies of the tomato. Genetics 39, 640–666 (1954)

  21. Rick, C.M.; Barton, D.W.: Cytogenetics of the tomato. Adv. Genet. 8, 267–371 (1956)

  22. Rick, C.M.; Notani, N.K.: The tolerance of extra chromosomes by primitive tomatoes. Genetics 46, 1231–1235 (1961)

  23. Sree Ramulu, K.; Devreux, M.; Ancora, G.; Laneri, U.: Chimerism in Lycopersicum peruvianum plants regenerated from in vitro cultures of anthers and stem internodes. Z. Pflanzenzüchtg. 76, 299–316 (1976)

  24. Tsuchiya, T.: Cytogenetic studies of trisomics in barley. Jap. J. Bot. 17, 177–213 (1960)

  25. Tsuchiya, T.: Establishment of trisomic series in a two rowed cultivated variety of barley. Can. J. Genet. Cytol. 9, 667–682 (1967)

  26. Vogt, G.E.; Rowe, P.R.: Aneuploids from triploid diploid crosses in the series Tuberosa of the Solanum. Can. J. Genet. Cytol. 10, 479–486 (1968)

Download references

Author information

Additional information

This work has been supported by a contract between the European Communities and the CNEN. This publication is contribution no. 1458 from the Biology Division of the European Communities and contribution no. 472 from the Divisione Applicazioni delle Radiazioni del CNEN.

Communicated by H.F. Linskens

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sree Ramulu, K., Carluccio, F., de Nettancourt, D. et al. Trisomics from triploid-diploid crosses in self-incompatible Lycopersicum peruvianum . Theoret. Appl. Genetics 50, 105–119 (1977). https://doi.org/10.1007/BF00276804

Download citation

Key words

  • Lycopersiaum peruvianum
  • Aneuploids
  • Trisomics
  • Self compatibility
  • Specificities