Amino acid analysis has shown that Lithosperum erythrorhizon cell suspension cultures which are unable to produce shikonin derivatives in LS medium containing ammonium accumulate a large quantity of glutamine, as compared with shikonin-producing cells cultured in the production medium M9 containing nitrate as the sole nitrogen source. The addition of glutamine to M9 medium proved to be strongly inhibitory to shikonin production. Furthermore, culture experiments using an inhibitor of glutaminase suggested that shikonin synthesis is not inhibited by ammonium released from glutamine but by glutamine itself. These findings indicate that the repression of shikonin synthesis occurs in close association with an accumulation of glutamine in cultured cells grown in a medium containing ammonium.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Fujita Y, Hara Y, Ogino T, Suga C (1981 a) Plant Cell Reports 1, 59–60.
Fujita Y, Hara Y, Suga C, Morimoto T (1981 b) Plant Cell Reports 1, 61–63.
Fukui H, Yoshikawa N, Tabata M (1983) Phytochemistry 22, 2451–2435.
Hartman SC (1968) J. Biol. Chem. 243, 853–863.
Linsmaier EF, Skoog F (1965) Physiol. Plant. 18, 100–127.
Mizukami H, Konoshima M, Tabata M (1978) Phytochemistry 17, 95–97.
Tabata M, Mizukami H, Hiraoka N, Konoshima M (1974) Phytochemistry 13, 927–932.
White PM (1963) The Cultivation of Animal and Plant Cells (2nd ed.) Ronald Press, New York.
Communicated by F. Constabel
About this article
Cite this article
Yazaki, K., Fukui, H., Kikuma, M. et al. Regulation of shikonin production by glutamine in Lithospermum erythrorhizon cell cultures. Plant Cell Reports 6, 131–134 (1987). https://doi.org/10.1007/BF00276670
- Cell Suspension