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Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue

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Abstract

A reproducible method for regeneration of plants from primary leaf tissue of 27 varieties of soybean (Glycine max), encompassing maturity groups 00 to VIII, has been developed. Progeny from seeds recovered from regenerated plants appear normal. Best regeneration was from leaf explants (2.1–4.0 mm) obtained from 5 day old seedlings. While 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was demonstrated to be essential for regeneration, addition of benzyladenine (BA) was found to enhance regeneration. Of the 6 other auxins tested, only picloram induced any regenerative response. Using identical volumes of medium and other conditions, regeneration could be obtained in 95 × 25 mm glass culture tubes but not in 60 × 15 mm Petri dishes.

The regeneration of soybeans from primary leaf tissue was shown to be greatly enhanced by pyroglutamic acid (5-oxoproline). Stimulatory effects were attained if pyroglutamic acid was added directly to the medium or if it was formed in situ as a result of chemical transformation of glutamine during autoclaving. The “active” component produced by autoclaving glutamine was not a conjugate of glutamine with inorganic salts or another organic component of the medium. Filter-sterilized glutamine was shown to be inhibitory to regeneration.

Murashige and Skoog (MS) and Schenk and Hildebrandt (SH) basal media were compared to Gamborg B5 medium. All contained 0.1 mg/l 2,4,5-T, 40 mg/l adenine sulfate and 10 mM pyroglutamic acid. No regeneration occurred when MS medium was used. Growth and appearance of callus growing on SH and B5 media with the additives were similar. The incidence of regeneration among cultures growing on SH medium was only one third compared to cultures grown on B5 medium.

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References

  1. Barwale UB, Kerns HR, Widholm JM (1986) Planta 167:473–481

  2. Christianson ML, Warnick DA, Carlson PS (1983) Science 222:632–634

  3. Delannay, X (1986) Unpublished data

  4. Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158

  5. Gamborg OL, Murashige T, Thorpe TA, Vasil IK (1976) In Vitro 12:473–478

  6. Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) Science 227:1229–1231

  7. Kameya T, Widholm J (1981) Plant Sci Lett 21:289–294

  8. Kao KN, Michayluk MR (1981) In Vitro 17:645–648

  9. Kimball SL, Bingham ET (1973) Crop Sci 13:758–760

  10. Larsen P (1980) In The Biochemistry of Plants — A Comprehensiven Treatise. Academic Press, NY 5:251

  11. Lippmann G, Lippmann G (1984) Pl Cell Rep 3:213–218

  12. McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Pl Cell Rep 5:81–84

  13. Mroginski LA, Kartha KK (1981) Pl Cell Rep 1:64–66

  14. Murashige T, Skoog F (1962) Physiol Plant 15:473–497

  15. Phillips GC, Collins GB (1979) Crop Sci 19:59–64

  16. Phillips GC, Collins GB (1981) Pl Cell Tiss Org Cult 1:123–129

  17. Ranch JP, Oglesby L, Zielinski AC (1985) In Vitro Cell Dev Biol 21:653–658

  18. Reynolds BD, Blackmon WJ, Lawrence AM (1982) Hort Sci 17:60

  19. Sator C (1985) Pl Cell Rep 4:126–128

  20. Schenk RV, Hildebrandt AC (1972) Can J Bot 50:199–204

  21. Tritsch GL, Moore GE (1962) Exp Cell Res 28:260–264

  22. Wright MS, Koehler SM, Hinchee MA, Carnes MG (1986a) Pl Cell Rep 5:150–154.

  23. Wright MS, Williams MH, Pierson, PE, Carnes MG (1986b) Pl Cell Tiss Org Cult (in press)

  24. Yang CT, Chen CK, Liu CM, Chang CM (1984) KOH SHUEH TUNG PAO (Science Bulletin) 16:1012–1016

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Author information

Correspondence to M. S. Wright.

Additional information

Communicated by I. K. Vasil

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Wright, M.S., Ward, D.V., Hinchee, M.A. et al. Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue. Plant Cell Reports 6, 83–89 (1987). https://doi.org/10.1007/BF00276659

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Keywords

  • Glutamine
  • Inorganic Salt
  • Leaf Explants
  • Benzyladenine
  • Maturity Group