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Somatic embryogenesis and plantlet regeneration in Cornus florida

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Abstract

Somatic embryos were initiated from 12 to 15 weeks postanthesis (WPA) zygotic embryos of Cornus florida L. (flowering dogwood) cultured on Murashige-Skoog (MS) or Schenk and Hildebrandt (SH) medium amended with either 3 mg/L 2,4-D or 5 mg/L 2,4-D and 1 mg/L kinetin. White, opaque globular and early cotyledonary stage embryos were formed directly on detached cotyledons from 2 of the 5 trees sampled after 7 weeks of culture. Morphologically mature embryos developed after an additional 4 weeks incubation on medium without growth regulators; however, many of the embryos were fused in pairs along the entire length of the hypocotyl-radicle axis. Indirect embryogenesis was observed from callus cultures initiated from 9 to 15 WPA zygotic embryos. These cultures have continued to produce embryos for 16 months. Many of the embryos formed roots on germination medium, but only 12% formed plantlets and none developed past the first true leaf stage.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

6-benzylaminopurine

NAA:

1-naphthaleneacetic acid

FPA:

Formalin-propionic acid-ethanol (50%)

WPA:

weeks post-anthesis

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Author information

Correspondence to Robert N. Trigiano.

Additional information

Communicated by J. M. Widholm

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Trigiano, R.N., Beaty, R.M. & Dietrich, J.T. Somatic embryogenesis and plantlet regeneration in Cornus florida. Plant Cell Reports 8, 270–273 (1989). https://doi.org/10.1007/BF00274127

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Keywords

  • Somatic Embryo
  • Somatic Embryogenesis
  • Kinetin
  • Callus Culture
  • Zygotic Embryo