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Molecular and General Genetics MGG

, Volume 226, Issue 1–2, pp 277–282 | Cite as

Genetic studies of the PRP11 izene of Saccharomyces cerevisiae

  • Keith Schappert
  • James D. Friesen
Article

Summary

PRP11 is a gene that encodes an essential function for pre-messenger RNA (mRNA) processing in Saccharomyces cerevisiae. We have carried out a mutational study to locate essential and non-essential regions of the PRP11 protein. The existing temperature-sensitive (ts) mutation (prpl1-1) was isolated from the chromosome of the original mutant and its position in the gene was determined. When the prp11-1 gene was transcribed from the GAL1 promoter, the overproduced protein was able to reverse the is prp11-1 phenotype; this is compatible with the possibility that the defect in the prp11-1 gene product affects its binding to the spliceosome. Thirteen linker-insertion mutations were constructed. Only five (prp11-4, 11-6, 11-10, −13 and −14) resulted in a null phenotype. One of these became temperature-sensitive when the insertion was.reduced in size from four (prp11-10) to two (prp11-15) amino acids. A sequence of ten amino acids of which also occurs in the human U1 small nuclear ribonucleoprotein particle (snRNP) A protein and the U2 snRNP B″ protein, when deleted from PRP11, had no phenotype and thus appears to be nonessential for PRP11 function. However, a linker-insertion mutation (prp11–10) immediately adjacent to this region resulted in a null phenotype.

Key words

mRNA processing Linker-insertion mutagenesis 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Keith Schappert
    • 1
    • 2
  • James D. Friesen
    • 1
    • 2
  1. 1.Department of GeneticsThe Hospital for Sick ChildrenTorontoCanada
  2. 2.Department of Medical GeneticsUniversity of TorontoTorontoCanada

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