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Genetic and molecular characterization of an Adh-1 null mutant in tomato

Summary

Treatment of tomato seeds with ethyl methanesulphonate (EMS) followed by allyl alcohol selection of M2 seeds has led to the identification of one plant (B15-1) heterozygous for an alcohol dehydrogenase (Adh) null mutation. Genetic analysis and expression studies indicated that the mutation corresponded to the structural gene of the Adh-1 locus on chromosome 4. Homozygous Adh-1 null mutants lacked ADH-1 activity in both pollen and seeds. Using an antiserum directed against ADH from Arabidopsis thaliana, which crossreacts with ADH-1 and ADH-2 proteins from tomato, no ADH-1 protein was detected in seeds of the null mutant. Northern blot analysis showed that Adh-1 mRNA was synthesized at wild-type levels in immature seeds of the null mutant, but dropped to 25% in mature seeds. Expression of the Adh-2 gene on chromosome 6 was unaffected. The potential use of the Adh-1 null mutant in selecting rare transposon insertion mutations in a cross with “mutable” Adh-1 + tomato lines is discussed.

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Correspondence to P. Zabel.

Additional information

Communicated by H. Saedler

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Wisman, E., Koornneef, M., Chase, T. et al. Genetic and molecular characterization of an Adh-1 null mutant in tomato. Mol Gen Genet 226, 120–128 (1991). https://doi.org/10.1007/BF00273595

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Key words

  • Lycopersicon esculentum
  • Ethyl methanesulphonate (EMS) treatment
  • Allyl alcohol selection
  • Alcohol dehydrogenase (ADH)
  • Adh-1 null mutant