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On the number of ribosomal RNA Genes in man

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Summary

The saturation plateau of the high molecular weight species of ribosomal RNA was determined for DNA of human liver from a female and a male person with normal karyotypes. The amount of DNA annealing with (18s+28s) RNA of HeLa cells is equivalent to 443±21 or 416±19 copies of the corresponding gene loci, depending on the set of molecular weights of 18s and 28s RNA used in the calculation.

Zusammenfassung

Es wurden RNA-DNA-Hybridisierungsexperimente mit DNA aus der Leber einer Frau und eines Mannes mit normalem Karyotyp und dem Gemisch der beiden hochmolekularen Komponenten der ribosomalen RNA durchgeführt. Der Anteil der menschlichen DNA, der mit (18s+28s) RNA aus HeLa-Zellen hybridisiert, entspricht 443±21 bzw. 416±19 Kopien der für diese RNA-Species codierenden Gene, je nach der Größe der Molekulargewichte, die für die Berechnung verwendet werden.

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Supported by the Deutsche Forschungsgemeinschaft (SFB 46).

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Bross, K., Krone, W. On the number of ribosomal RNA Genes in man. Humangenetik 14, 137–141 (1972). https://doi.org/10.1007/BF00273298

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Keywords

  • Molecular Weight
  • Internal Medicine
  • HeLa Cell
  • High Molecular Weight
  • Metabolic Disease