Human Genetics

, Volume 70, Issue 2, pp 163–167 | Cite as

Subtyping of haptoglobin — Presentation of a new method

  • Brita Teige
  • Bjørnar Olaisen
  • Lilian Pedersen
Original Investigations


A method is described for large scale routine phenotyping of haptoglobin (Hp) which allows complete subtyping without prior purification of the Hp molecule. The procedure includes polyacrylamide gel isoelectric focusing of reduced, neuraminidase treated serum or plasma samples, and nitrocellulose blots developed with the immunoperoxidase technique. Different variables including sample treatment, electrofocusing, blotting procedures, and immunoperoxidase visualization are discussed.

Characteristic α-chain patterns allow identification of the common allotypes 2FS, 2SS, 2FF, IS, IF, and Johnson. Isoelectric variations in the β-chain may also be recognized. For comparison, two-dimensional Hp-patterns are presented. The results from concurrent typing of 600 samples by ordinary starch gel electrophoresis and by the described isofocusing technique, are evaluated.


Starch Polyacrylamide Metabolic Disease Plasma Sample Sample Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Brita Teige
    • 1
  • Bjørnar Olaisen
    • 1
  • Lilian Pedersen
    • 1
  1. 1.Institute of Forensic MedicineUniversity of Oslo, RikshospitaletOslo 1Norway

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