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Plant Cell Reports

, Volume 7, Issue 1, pp 59–62 | Cite as

Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones

  • Julie A. Russell
  • Brent H. McCown
Article

Abstract

Leaf protoplasts were isolated from shoot cultures of two hybrid poplar clones (Populus alba × P. grandidentata ‘Crandon’, NC-5339 and P. nigra ‘Betulifolia’ × P. trichocarpa, NC-5331) and the Upright European Aspen (P. tremula ‘Erecta’) and were cultured in contact with screen discs floated in liquid medium. Protoplast culture was influenced by the growth medium of the source shoot cultures, the protoplast purification procedure, the plating density, and the presence or absence of a coconut water and casein hydrolysate supplement added to the culture medium. The protoplast-derived cells divided more quickly and with higher incidence than previously reported for hybrid poplars. Shoots were regenerated from the protoplast-derived calli and were maintained as shoot cultures. Plants were developed from microcuttings rooted ex vitro and were grown-on in the greenhouse and field.

Keywords

Growth Medium Liquid Medium Alba Purification Procedure Casein Hydrolysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BA

benzyladenine

NAA

1-naphthaleneacetic acid

TDZ

thidiazuron

WPM

Woody Plant Medium, Lloyd and McCown (1980)

MS

Murashige and Skoog Medium (1962)

NC-XXXX

North Central Forest Experiment Station accession number assigned to hybrid poplar clones.

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Julie A. Russell
    • 1
  • Brent H. McCown
    • 1
  1. 1.Department of HorticultureUniversity of WisconsinMadisonUSA

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