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Stimulation of chalcone synthase activity by yeast extract in cultured Glycyrrhiza echinata cells and 5-deoxyflavanone formation by isolated protoplasts


Chalcone synthase activity catalyzing the formation of naringenin (5-hydroxyflavanone) was detected in cell suspension cultures of Glycyrrhiza echinata. This activity rapidly increased by treatment of the cells with yeast extract, while non-treated cells showed a constant low activity. Isolated G. echinata protoplasts accumulated retrochalcone (echinatin) and its biosynthetic intermediate (licodione) during 24 h of culture. When the protoplasts were incubated with [14C(U)]phenylalanine, liquiritigenin (5-deoxyflavanone) was transiently labeled, indicating the induction of 6'-deoxychalcone synthase. The formation of liquiritigenin, in addition to naringenin, was observed when the crude extracts from the protoplasts were assaved for CHS activity.

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chalcone synthase


yeast extract


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Correspondence to T. Furuya.

Additional information

This paper is Part 52 in the series “Studies on Plant Tissue Cultures”. For Part 51, see Furuya T, Ushiyama M, Asada Y, Yoshikawa T, Orihara Y (1987) Phytochemistry: in press.

Communicated by S. Ayabe

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Ayabe, S., Udagawa, A. & Furuya, T. Stimulation of chalcone synthase activity by yeast extract in cultured Glycyrrhiza echinata cells and 5-deoxyflavanone formation by isolated protoplasts. Plant Cell Reports 7, 35–38 (1988).

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  • Cell Suspension
  • Yeast Extract
  • Phenylalanine
  • Crude Extract
  • Suspension Culture