Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12

  • 33 Accesses

  • 61 Citations

Summary

Mutants carrying recF143 or recF144 show wild type levels of host cell reactivation of UV-irradiated λvir and wild type rates of excision gap closure in repairing UV damage to their own DNA. The same mutants showed reduced rates of postreplication repair strand joining. When uvrA - recF- or uvrB - recF- strains are tested, postreplication repair strand joining is incomplete or does not occur at fluences above 1 J/m2. We suggest that there may be a UvrAB and a RecF pathway of postreplication repair or that the repair functions controlled or determined by uvrA uvrB and by recF may be similar. An intermediate in postreplication repair may accumulate in the uvr - recF- strain.

This is a preview of subscription content, log in to check access.

References

  1. Bachmann, B.J.: Pedigrees of some mutant strains of Escherichia coli K12. Bact. Rev. 36, 525–557 (1972)

  2. Bachmann, B.J., Low, K.B., Taylor, A.L.: Recalibrated linkage map of Escherichia coli K-12. Bact. Rev. 40, 116–167 (1976)

  3. Bridges, B.A.: Recent advances in basic mutation research. Address to 6th annual meeting of the European Environmental Mutagen Society at Gernrode, October, 1976 (1977)

  4. Demerec, M., Adelberg, E.A., Clark, A.J., Hertman, P.E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966)

  5. Ganesan, A.K.: Persistence of pyrimidine dimers during postreplication repair in ultraviolet-light irradiated Escherichia coli K-12. J. molec. Biol. 87, 103–119 (1974)

  6. Ganesan, A.K., Seawell, P.C.: The effect of lexA and recF mutations on postreplication repair and DNA synthesis in Escherichia coli K-12. Molec. gen. Genet. 141, 189–205 (1975)

  7. Gellert, M., Mizuuchi, K., O'Dea, H., Nash, H.: DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proc. nat. Acad. Sci. (Wash.) 73, 3872–3876 (1976a)

  8. Gellert, M., O'Dea, M.H., Itoh, T., Tomizawa, J.-I.: Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc. nat. Acad. Sci. (Wash.) 73, 4474–4478 (1976b)

  9. Horii, Z.-I., Clark, A.J.: Genetic analysis of the RecF pathway to genetic recombination in Escherichia coli K-12: isolation and characterization of mutants. J. molec. Biol. 80, 327–344 (1973)

  10. Howard-Flanders, P., Boyce, R.P., Theriot, L.: Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53, 1119–1136 (1966)

  11. Kato, T., Rothman, R.H., Clark, A.J.: Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation. Genetics (in press) (1977)

  12. Radman, M.: SOS repair hypothesis: phenomenology of an inducible repair which is accompanied by mutagenesis. In: Molecular mechanisms for repair of DNA (P.C. Hanawalt and R.B. Setlow, eds.), pp. 355–367. New York: Plenum Press 1975

  13. Rosner, J.L., Kass, L.R., Yarmolinsky, M.B.: Parallel behavior of F and P1 in causing indirect induction of lysogenic bacteria. Cold Spr. Harb. Symp. quant. Biol. 33, 785–789 (1968)

  14. Rothman, R.H., Clark, A.J.: Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12. (in press Molec. gen. Genet.) (1977)

  15. Rothman, R.H., Kato, T., Clark, A.J.: The beginning of an investigation of the role of recF in the pathways of metabolism of UV-irradiated DNA, in Escherichia coli. In: Molecular mechanisms for repair of DNA (P.C. Hanawalt and R.B. Setlow, eds.), pp. 291–293. New York: Plenum Press 1975

  16. Rupp, W.D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol. 31, 291–304 (1968)

  17. Sedliakova, M., Brozmanova, J., Slezarikova, B., Masek, F., Fandlova, E.: Function of the uvr marker in dark repair of DNA molecules. Neoplasma 22, 361–384 (1975)

  18. Shizuya, H., Dykhuizen, D.: Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I. J. Bact. 112, 676–681 (1972)

  19. Smith, K.C., Meun, D.H.C.: Repair of radiation induced damage in Escherichia coli I. Effect of rec mutations on postreplication repair of damage due to ultraviolet radiation. J. molec. Biol. 51, 459–472 (1970)

  20. Witkin, E.M.: Elevated mutability of polA and uvrA polA derivatives of Escherichia coli B/r at sublethal doses of ultraviolet light: evidence for an inducible error-prone repair system (“SOS repair”) and its anomalous expression in these strains. Genetics 79 (suppl.), 199–213 (1975)

Download references

Author information

Correspondence to Alvin J. Clark.

Additional information

Communicated by E. Witkin

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rothman, R.H., Clark, A.J. The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12. Molec. Gen. Genet. 155, 279–286 (1977). https://doi.org/10.1007/BF00272806

Download citation

Keywords

  • Escherichia Coli
  • Host Cell
  • Cell Reactivation
  • Type Rate
  • Wild Type Level