Molecular and General Genetics MGG

, Volume 177, Issue 4, pp 691–698 | Cite as

W-mutagenesis in competent cells of Bacillus subtilis

  • S. E. Bresler
  • V. L. Kalinin
  • R. A. Kreneva


The relative yield (Nm/N) of fluorescent mutants Ind- after the transformation of Bacillus subtilis cells by means of UV-irradiated DNA is much higher in an uvr- recipient than in an uvr+ strain, when compared at equal fluence, but practically identical at equal survival. Ind- mutations are induced by UV-irradiation of separated single strands of transforming DNA. The H-strand is much more sensitive to the mutagenic action of UV light. Preliminary irradiation of competent recipient cells by moderate UV fluences increases the survival of UV-or γ-irradiated transforming DNA (W-reactivation) and the frequency of Ind- mutations (W-mutagenesis). During transfection of B. subtilis cells by UV-irradiated prophage DNA isolated from lysogenic cells B. subtilis (Ø105 c+) c-mutants of the phage are obtained in high yield only in conditions of W-mutagenesis, i.e. in UV-irradiated recipient cells. These data show that there is no substantial spontaneous induction of error-prone SOS-repair system in the competent cells of B. subtilis.


Bacillus Bacillus Subtilis Competent Cell Single Strand Relative Yield 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • S. E. Bresler
    • 1
  • V. L. Kalinin
    • 1
  • R. A. Kreneva
    • 1
  1. 1.Leningrad Institute of Nuclear PhysicsAcademy of Sciences of the USSRLeningradUSSR

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