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Separation of two distinct S-adenosylmethionine dependent N-methyltransferases involved in hordenine biosynthesis in Hordeum vulgare

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Abstract

Hordenine is biosynthesized in young roots of barley by subsequent N-methylation of tyramine. It was shown that two distinct enzymes are responsible for these methylation reactions. They differed in their pH-optimum, their stability in dependence of the pH-value, and were partially resolved by DEAE-chromatography. More than 200-fold purification and almost complete separation were achieved by elution of the enzymes from an affinity column which was prepared by coupling S-adenosylhomocysteine to aminohexyl-Sepharose 4 B.

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Abbreviations

NMT:

N-methyltyramine

SAM:

S-adenosyl-L-methionine

SAH:

S-adenosyl-L-homocysteine

DTE:

dithioerythritol

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Meyer, E. Separation of two distinct S-adenosylmethionine dependent N-methyltransferases involved in hordenine biosynthesis in Hordeum vulgare . Plant Cell Reports 1, 236–239 (1982). https://doi.org/10.1007/BF00272627

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Keywords

  • Tyramine
  • Complete Separation
  • Affinity Column
  • Methylation Reaction
  • Young Root