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Plant regeneration in zigzag clover (Trifolium medium L.)

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A study was conducted to regenerate plants from explant tissue and from callus culture of zigzag clover (Trifolium medium). Petiole segments from two strains of zigzag clover were cultured on L2 or in SL2 media. Shoots were regenerated via organogenesis from petiole segments of both strains of zigzag clover. Direct shoot regeneration was noticed as early as eight days after the initiation of cultures. Shoots were also regenerated via somatic embryogenesis from petiole-derived calli of the two strains on L2 and SL2 media. Regenerated plants have normal morphological characteristics.

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  1. Broertjes C, Roest S, Bokelmann GS (1976) Euphytica 26: 11–19.

  2. Collins, GB, Phillips GC (1982) In: Earle ED, Demarly Y (eds.), Regeneration from cells and tissue culture. Praeger Scientific Press, NY p. 22–34.

  3. Faust N, Gasser H (1980) Crop Sci. 20: 417.

  4. Grosser JW, Collins GB (1984) Plant Sci. Lett. 37: 165–170.

  5. Keim WF (1953) Agron J. 45: 601–606.

  6. Maheswaran CT, Williams EG (1984) Annals of Botany 54: 201–211.

  7. Merker A (1984) Hereditas 101: 267–268.

  8. Parrott WA, Collins GB (1983) Plant Sci. Lett. 28: 189–194.

  9. Phillips GC, Collins GB, Taylor NL (1982) Theor. Appl. Genet. 62: 17–24.

  10. Robertson RW (1966) Can. Agric. 11: 8–9.

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Communicated by F. Constabel

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Choo, T.M. Plant regeneration in zigzag clover (Trifolium medium L.). Plant Cell Reports 7, 246–248 (1988). https://doi.org/10.1007/BF00272534

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  • Morphological Characteristic
  • Somatic Embryogenesis
  • Plant Regeneration
  • Shoot Regeneration
  • Callus Culture