Wild-type plants of Arabidopsis thaliana strain “Columbia” regenerated at a high frequency from immature cotyledons cultured on a shoot-inducing medium containing 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l 1-naphthaleneacetic acid. Cotyledon segments expanded rapidly and produced numerous shoots after 2–3 weeks in culture. Regeneration occurred in the absence of the original shoot apex. Hypocotyl segments from immature embryos produced root hairs and callus in culture but only rarely developed shoots. Hygromycin, kanamycin and G-418 inhibited cotyledon expansion and shoot formation in culture. Vancomycin was much less toxic to cotyledon segments than either carbenicillin or cefotaxime. Immature cotyledons therefore yield numerous regenerated plants that may be useful in future transformation studies.
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Acedo GN (1986) Plant Cell Tissue Organ Cult 6:109–114
An G, Watson BD, Chiang CC (1986) Plant Physiol 81:301–305
Baus AD, Franzmann L, Meinke DW (1986) Theor Appl Genet 72:577–586
Duncan DR, Williams ME, Zehr BE, Widholm JM (1985) Planta 165:322–332
Estelle M, Somerville CR (1986) Trends in Genet 2:89–93
Feldmann KA, Marks MD (1986) Plant Sci 47:63–69
Feldmann KA, Marks MD (1987) Mol Gen Genet 208:1–9
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158
Gotto N (1979) Jpn J Genet 54:303–306
Heath JD, Weldon R, Monnot C, Meinke DW (1986) Planta 169:304–312
Huang BC, Yeoman MM (1984) Plant Sci Lett 33:353–363
Lloyd AM, Barnason AR, Rogers SG, Byrne MC, Fraley RT, Horsch RB (1986) Science 234:464–466
Marsden MPF, Meinke DW (1985) Amer J Bot 72:1801–1812
Meinke DW (1985) Theor Appl Genet 69:543–552
Meinke DW (1986) Oxford Surv Plant Mol Cell Biol 3:122–165
Meinke DW, Sussex IM (1979) Dev Biol 72:50–61
Meyerowitz EM, Pruitt RE (1985) Science 229:1214–1218
Murashige T, Skoog F (1962) Physiol Plant 15:493–497
Negrutiu I, Jacobs M (1978) Z Pflanzenphysiol 90:423–430
Negrutiu I, Jacobs M, de Greef W (1978) Z Pflanzenphysiol 90:363–372
Patton DA (1986) M.S. Thesis, Oklahoma State Univ
Ranch JP, Oglesby L, Zielinsk AC (1985) In Vitro 21:653–658
Rogers SG, Horsch RB, Fraley RT (1986) Methods Enzymol 118:627–640
Skvirksy RC, Hanson MR, Ausubel FM (1984) Plant Sci Lett 35:237–246
Zhang H, Somerville CR (1987) Plant Sci 48:165–173
Communicated by G. C. Phillips
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Patton, D.A., Meinke, D.W. High-frequency plant regeneration from cultured cotyledons of Arabidopsis thaliana . Plant Cell Reports 7, 233–237 (1988). https://doi.org/10.1007/BF00272531
- Root Hair
- Shoot Apex